College of Chemistry, Green Catalysis Center, Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, Zhengzhou University, Zhengzhou 450001, China.
ACS Appl Mater Interfaces. 2020 Jul 15;12(28):31738-31744. doi: 10.1021/acsami.0c05005. Epub 2020 Jul 1.
Carbon dots (CDs) have been widely studied for their excellent properties. However, most of the prepared CDs only show strong emission in the blue to green region, which greatly limits the application of CDs in the biomedical field. In this report, a new design strategy of long-wavelength CDs was reported. The orange phenyl-CDs with good optical properties and biocompatibility were successfully prepared by changing the substituted group of the -phenylenediamine and the main emission band of phenyl-CDs was in the far-red region. With the increase of polarity, the wavelength of phenyl-CDs red-shifts and the fluorescence intensity decreases, demonstrating their sensitive polarity response function. In addition, phenyl-CDs can achieve ultrafast target imaging of lysosome within 40 s through clathrin-mediated endocytosis. Finally, phenyl-CDs were successfully applied for monitoring lysosomal polarity induced by drugs, which is helpful in getting a better understanding of the physiological and pathological processes of lysosomes. This report provides an important theoretical basis for the rational design and precise synthesis of long-wavelength fluorescent CDs.
碳点 (CDs) 因其优异的性能而得到广泛研究。然而，大多数制备的 CDs 仅在蓝绿光区域显示强发射，这极大地限制了 CDs 在生物医学领域的应用。在本报告中，报道了一种长波长 CDs 的新设计策略。通过改变 -苯二胺的取代基，成功制备出具有良好光学性能和生物相容性的橙色苯-CDs，苯-CDs 的主发射带位于远红区。随着极性的增加，苯-CDs 的波长红移，荧光强度降低，表现出其对极性的敏感响应功能。此外，苯-CDs 可以通过网格蛋白介导的内吞作用在 40 秒内实现溶酶体的超快靶向成像。最后，苯-CDs 成功应用于监测药物诱导的溶酶体极性，这有助于更好地了解溶酶体的生理和病理过程。本报告为长波长荧光 CDs 的合理设计和精确合成提供了重要的理论基础。
