Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, College of Chemistry, Zhengzhou University, Zhengzhou, 450001, PR China.
Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, College of Chemistry, Zhengzhou University, Zhengzhou, 450001, PR China.
Anal Chim Acta. 2020 Apr 15;1106:207-215. doi: 10.1016/j.aca.2020.02.002. Epub 2020 Feb 4.
In this work, a novel bright yellow fluorescent carbon dots (CDs) was synthesized from N-methyl-1,2-phenylenediamine hydrochloride in ethanol solvent through the solvothermal method. The obtained carbon dots had no fluorescence in water, but could specifically light up in the cell which makes the staining process without washing. Interestingly, the imaging process can be performed by simply shaking the culture with the cells for only 1 min, indicating ultrafast and easy to operate fluorescence imaging. Moreover, the carbon dots with abundant amino functional groups had good lysosome targeting properties (the Pearson's correlation coefficient is 0.92) and could achieve the ultrafast lysosome imaging in cell and zebrafish and monitoring cell apoptosis status. This is the first lysosome targeting carbon dots that allows the combination of a short incubation at the second-level with wash-free process providing great potential for continuous observation lysosome in vivo.
在这项工作中，我们通过溶剂热法，以 N-甲基-1,2-苯二胺盐酸盐为原料，在乙醇溶剂中合成了一种新型亮黄色荧光碳点（CDs）。所得到的碳点在水中没有荧光，但可以在细胞中特异性点亮，从而实现无需洗涤的染色过程。有趣的是，只需简单地将细胞培养物摇晃 1 分钟即可进行成像过程，这表明其具有超快和易于操作的荧光成像性能。此外，具有丰富氨基官能团的碳点具有良好的溶酶体靶向特性（皮尔逊相关系数为 0.92），可以实现细胞和斑马鱼中超快速的溶酶体成像，并监测细胞凋亡状态。这是第一个允许在二级孵育过程中与免洗过程相结合的溶酶体靶向碳点，为在体内持续观察溶酶体提供了巨大的潜力。
