Clinic of Infectious Diseases, University of Bari, University Hospital Policlinico, Bari, Italy.
J Med Virol. 2020 Dec;92(12):3271-3278. doi: 10.1002/jmv.26246. Epub 2020 Jul 11.
HIV-1 V2 domain binds α4β7, which assists lymphocyte homing to gut-associated lymphoid tissue. This triggers bacterial translocation, thus contributing to immune activation. We investigated whether variability of V2 binding site could influence plasma levels of lipopolysaccharide (LPS) and soluble cluster of differentiation 14 (sCD14), markers of microbial translocation/immune activation. HIV gp120 sequences from antiretroviral naïve patients were analyzed for V2 tripeptide composition, length, net charge, and potential N-linked-glycosylation sites. LPS and sCD14 plasma levels were quantified. Clinical/immuno-virologic data were retrieved. Overall, 174 subjects were enrolled, 8% with acute infection, 71% harboring a subtype B. LDV was detected in 41% and LDI in 27%. No difference was observed between levels of LPS or sCD14 according to different mimotopes or according to other sequence characteristics. By multivariable analysis, only acute infection was significantly associated with higher sCD14 levels. In conclusion, no association was observed between V2 tripeptide composition and extent of bacterial translocation/immune activation.
HIV-1 V2 结构域与 α4β7 结合，协助淋巴细胞归巢至肠道相关淋巴组织。这会引发细菌易位，从而导致免疫激活。我们研究了 V2 结合位点的变异性是否会影响脂多糖（LPS）和可溶性分化簇 14（sCD14）的血浆水平，这些标志物可反映微生物易位/免疫激活。对接受抗逆转录病毒治疗的初治患者的 HIV gp120 序列进行 V2 三肽组成、长度、净电荷和潜在 N-糖基化位点分析。定量检测 LPS 和 sCD14 的血浆水平，并检索临床/免疫病毒学数据。总体而言，共纳入 174 例患者，8%为急性感染，71%携带 B 亚型。41%的患者检测到 LDV，27%的患者检测到 LDI。不同模拟表位或其他序列特征之间，LPS 或 sCD14 的水平没有差异。多变量分析显示，只有急性感染与 sCD14 水平升高显著相关。总之，V2 三肽组成与细菌易位/免疫激活的程度之间没有关联。
