Department of Microbiology, All India Institute of Medical Sciences, India.
Department of Paediatrics, All India Institute of Medical Sciences, India.
Microb Pathog. 2017 Oct;111:331-337. doi: 10.1016/j.micpath.2017.08.004. Epub 2017 Aug 9.
Microbial translocation of lipopolysaccharides (LPS), soluble CD14 (sCD14) and IgM Endocab levels have been reported to be associated with disease progression in HIV-1 infection. In this longitudinal study, plasma levels of different microbially translocated products (LPS, sCD14, Endocab) was investigated in HIV-1 infected Indian Individuals stratified as Rapid (R), Viremic slow (VS), Slow progressors (S) and healthy controls.
Ten healthy and twenty HIV-1 infected individuals were enrolled. Plasma levels of LPS, sCD14, Endocab was examined using commercially available Limulus Amebocyte assay and enzyme-linked immunosorbent assay (ELISA) enzyme linked immunosorbant assay.
Elevated levels of sCD14, IgM EndoCab and LPS were observed during HIV-1 infection compared to healthy controls. Rapid progressors had higher levels of sCD14, IgM EndoCab, LPS (median% 1553, 3596, 202.2) compared to viremic slow, slow progressors and healthy controls both at baseline and follow up visits. At baseline, LPS correlated positively with IgM Endocab and negatively with sCD14 levels while at follow-up, significant positive correlation was observed between IgM Endocab and sCD14 (IgM EndoCab r = 0.490, p = 0.05; sCD14 r = 0.051, p = 0.830). Plasma levels of sCD14 correlated positively with viral load in rapid, viremic slow and slow progressors while CD + T cell count correlated positively with sCD14 and IgM EndoCab levels in viremic slow and slow progressors.
Our findings indicate that elevated levels of sCD14, IgM EndoCab and LPS in HIV-1 infected individuals are strong predictors of disease progression and could be considered as candidate biomarkers for disease monitoring.
脂多糖（LPS）、可溶性 CD14（sCD14）和 IgMEndocab 的微生物易位已被报道与 HIV-1 感染中的疾病进展相关。在这项纵向研究中，对不同微生物易位产物（LPS、sCD14、Endocab）的血浆水平进行了研究，研究对象为 HIV-1 感染的印度个体，分为快速进展者（R）、病毒血症缓慢进展者（VS）、缓慢进展者（S）和健康对照者。
纳入了 10 名健康人和 20 名 HIV-1 感染者。使用商用鲎阿米巴原虫检测和酶联免疫吸附试验（ELISA）酶联免疫吸附试验检测 LPS、sCD14、Endocab 的血浆水平。
与健康对照组相比，HIV-1 感染期间 sCD14、IgMEndocab 和 LPS 水平升高。快速进展者的 sCD14、IgMEndocab、LPS 水平高于病毒血症缓慢进展者、缓慢进展者和健康对照组，基线和随访时均如此（中位数% 1553、3596、202.2）。基线时，LPS 与 IgMEndocab 呈正相关，与 sCD14 呈负相关，而随访时，IgMEndocab 与 sCD14 之间呈显著正相关（IgMEndocab r=0.490，p=0.05；sCD14 r=0.051，p=0.830）。快速进展者、病毒血症缓慢进展者和缓慢进展者的 sCD14 血浆水平与病毒载量呈正相关，而 CD+T 细胞计数与病毒血症缓慢进展者和缓慢进展者的 sCD14 和 IgMEndocab 水平呈正相关。
我们的发现表明，HIV-1 感染者中 sCD14、IgMEndocab 和 LPS 水平升高是疾病进展的强预测因子，可作为疾病监测的候选生物标志物。
