Shan Yongli, Ma Lishi, Zhang Cong, Zhang Yanqi, Zhang Jingyuan, Xiao Lu, Wei Yanxing, Yu Yanhong
Placenta Research Group, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
Placenta Research Group, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Stem Cell Res. 2020 Jun 24;47:101885. doi: 10.1016/j.scr.2020.101885.
Human RNF2 (RING1B) gene is a critical epigenetic modification factor for embryonic development, pluripotency and differentiation of embryonic stem cells (ESCs). To further gain insights into the role of RNF2 in cell fate decisions of human ESCs, here we generated two RNF2 homozygous knockout human ESC lines by CRISPR/Cas9 genome editing technology. These cell lines maintained a normal karyotype and typical undifferentiated state in terms of morphology, pluripotent gene expression, and had differentiation potential in vivo. These cell lines provide good cell resources to explore the role of RNF2 gene in embryonic development and lineage differentiation in vitro.
人类RNF2（RING1B）基因是胚胎发育、胚胎干细胞（ESC）多能性和分化的关键表观遗传修饰因子。为了进一步深入了解RNF2在人类ESC细胞命运决定中的作用，我们在此通过CRISPR/Cas9基因组编辑技术构建了两个RNF2纯合敲除的人类ESC细胞系。这些细胞系在核型、形态、多能基因表达方面维持正常，且在体内具有分化潜能。这些细胞系为体外探索RNF2基因在胚胎发育和谱系分化中的作用提供了良好的细胞资源。
