Potential Anti-Tuberculosis Activity of the Extracts and Their Active Components of (DC.) Guill. and Perr. with Special Emphasis on Polyphenols.

In Sudanese traditional medicine, decoctions of the stem bark of are used for the treatment of tuberculosis (TB). However, this plant has not been investigated before for its antimycobacterial effects. Our screening results show, for the first time, that many extracts of various parts of exhibit growth inhibitory activity against . Minimum inhibitory concentration (MIC) values ranged between 625 and 5000 µg/mL, with an ethyl acetate extract of the root showing the lowest MIC value. The good antimycobacterial effects of the root part could be due to its high concentration of ellagic acid derivatives, ellagitannins, and flavonoids. Thin layer chromatography (TLC) fractionation resulted in some fractions with better activity than the starting point crude methanol extract (MIC 2500 µg/mL). Those fractions with the lowest MIC values contained a high number of antioxidant compounds. Fractions and (MIC 1500 and 1000 µg/mL, respectively) contained high concentrations of di-methyl ellagic acid ([M-H] 329.0318). Fraction (MIC 2000 µg/mL) contained a lower concentration of di-methyl ellagic acid and was not as growth inhibitory as fractions and . Moreover, in fraction , an acetylated ellagic acid derivative ([M-H] 343.0477) and di-methyl-ellagic acid xyloside ([M-H] 461.0739) were tentatively characterized. Di-methyl ellagic acid xyloside was also present in fraction and could strongly contribute to the antimycobacterial effect of this fraction. Additionally, protocatechuic acid ([M-H]-at m/z 153.0196) was present in fraction . Our antimycobacterial results obtained from this research justify the use of in Sudanese folk medicine against cough related to TB. Roots, stem bark, and leaves of are sources for new potent anti-TB drug lead compounds.