Department of Respiratory Medicine, Ningbo Medical Center Lihuili Hospital, Ningbo, Zhejiang, China.
Department of Thoracic Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.
Int J Biol Markers. 2020 Sep;35(3):29-35. doi: 10.1177/1724600820938385. Epub 2020 Jul 2.
Non-small cell lung cancer (NSCLC) remains the most common cause of human cancer-related death worldwide, and the present study aims to explore the roles of long non-coding (lnc)RNA ZEB2-AS1 in NSCLC and the related mechanism.
Quantitative real-time-polymerase chain reaction was performed to compare the expressions of ZEB2-AS1 in NSCLC cancer tissue and the adjacent non-tumorous tissues. The diagnostic and prognostic roles of ZEB2-AS1 in NSCLC were also evaluated by the receiver operating characteristic curve and the Kaplan-Meier survival analysis. NSCLC cell line A549 cells were transfected with ZEB2-AS1 siRNA, and the cell viability, migration, invasion, and epithelial-mesenchymal transition (EMT) of the ZEB2-AS1 siRNA group and control group were compared.
ZEB2-AS1 was significantly increased in NSCLC tissues. The knockdown of ZEB2-AS1 markedly inhibited the cell viability, migration, invasion, and EMT of A549 cells in vitro.
ZEB2-AS1 was up-regulated in NSCLC, and it may serve as a potential target for the diagnosis, prognosis, and treatment of NSCLC.
非小细胞肺癌（NSCLC）仍然是全球导致人类癌症相关死亡的最常见原因，本研究旨在探讨长链非编码（lnc）RNA ZEB2-AS1 在 NSCLC 中的作用及其相关机制。
通过实时定量聚合酶链反应比较 NSCLC 癌组织和相邻非肿瘤组织中 ZEB2-AS1 的表达。通过受试者工作特征曲线和 Kaplan-Meier 生存分析评估 ZEB2-AS1 在 NSCLC 中的诊断和预后作用。用 ZEB2-AS1 siRNA 转染 NSCLC 细胞系 A549 细胞，比较 ZEB2-AS1 siRNA 组和对照组的细胞活力、迁移、侵袭和上皮-间充质转化（EMT）。
ZEB2-AS1 在 NSCLC 组织中明显上调。ZEB2-AS1 的敲低显著抑制了 A549 细胞的体外细胞活力、迁移、侵袭和 EMT。
ZEB2-AS1 在 NSCLC 中上调，它可能成为 NSCLC 诊断、预后和治疗的潜在靶点。
