Godard C, Hannecart-Pokorni E
Ann Microbiol (Paris). 1977 Jan;128A(1):19-23.
The rfa-7 lipopolysaccharide core mutation carried by the R mutant F680, derived from Shigella flexneri F6S serotype 5b, has been mapped by conjugation and transduction experiments. The results show a chromosomal distance of about 1 min between rfa-7 and mtl. Such a position would be similar to those of rfa genes in Escherichia coli K12 and Salmonella typhi-murium LT2. Conjugational transfer of E. coli K12 rfa+ genes to mutant F680 restored S. flexneri O-specificity. Chemical analyses performed on the lipopolysaccharide of such a rfa+ hybrid suggest that this strain can attach the S. flexneri serotype 5b O-specific polysaccharide to the E. coli K12 core.
源自福氏志贺菌F6S血清型5b的R突变体F680所携带的rfa - 7脂多糖核心突变，已通过接合和转导实验进行了定位。结果显示rfa - 7与mtl之间的染色体距离约为1分钟。这样的位置与大肠杆菌K12和鼠伤寒沙门氏菌LT2中rfa基因的位置相似。将大肠杆菌K12的rfa +基因接合转移到突变体F680可恢复福氏志贺菌的O特异性。对这种rfa +杂种的脂多糖进行的化学分析表明，该菌株可以将福氏志贺菌血清型5b的O特异性多糖连接到大肠杆菌K12核心上。
