Solak Kazim, Yucel Istemi, Karaduman Z Okan, Arda Sena, Orak M Mufit, Midi Ahmet
Department of Orthopedics and Traumatology, Duzce Atatürk State Hospital, Duzce, Turkey.
Department of Orthopedics and Traumatology, Fatih Sultan Mehmet Training and Research Hospital, Istanbul, Turkey.
Eurasian J Med. 2020 Jun;52(2):145-152. doi: 10.5152/eurasianjmed.2019.19077. Epub 2020 Jun 2.
We used biomimetic scaffolds, chondral scaffolds, and microfractures to repair experimentally created osteochondral defects in rat knees and then compared the results of each method.
We used a total of 56 female Wistar albino rats. The rats were grouped into 4 groups, with 14 rats each: biomimetic scaffold, chondral scaffold, microfracture, and control groups. Cylindrical full-thickness osteochondral defects 2.5 mm in diameter and 2 mm in depth were drilled into the right knees with the rats under general anesthesia. The knees of all rats were operated again after 4 weeks. Biomimetic and chondral scaffolds were classified into two groups. Microfractures 0.5 mm in diameter and 0.8 mm in depth were created in the rats of the microfracture group. The control group received no treatment. All the rats were observed for 6 weeks and then sacrificed, with samples subjected to macroscopic and histopathological examinations.
The macroscopic and histopathological results in the biomimetic scaffold group differed significantly from those of the other treatment groups (p<0.05). When we compared the 3 treatment groups, the results of the chondral scaffold group were better than those of the microfracture group. The results of the microfracture group were somewhat better than those of the control group, but the result was not statistically significant (p>0.05).
Nanocomposite multilayer biomimetic scaffolds were better than chondral scaffolds and microfractures when used to treat osteochondral defects.
我们使用仿生支架、软骨支架和微骨折技术修复大鼠膝关节实验性制造的骨软骨缺损，然后比较每种方法的结果。
我们总共使用了56只雌性Wistar白化大鼠。大鼠被分为4组，每组14只：仿生支架组、软骨支架组、微骨折组和对照组。在全身麻醉下，在大鼠右膝钻直径2.5毫米、深度2毫米的圆柱形全层骨软骨缺损。4周后对所有大鼠的膝关节再次进行手术。仿生支架和软骨支架分为两组。在微骨折组大鼠中制造直径0.5毫米、深度0.8毫米的微骨折。对照组不接受治疗。所有大鼠观察6周后处死，对样本进行宏观和组织病理学检查。
仿生支架组的宏观和组织病理学结果与其他治疗组有显著差异（p<0.05）。当我们比较3个治疗组时，软骨支架组的结果优于微骨折组。微骨折组的结果略优于对照组，但差异无统计学意义（p>0.05）。
纳米复合多层仿生支架在治疗骨软骨缺损方面优于软骨支架和微骨折技术。
