Ayadi Rania, Sitterlé Emilie, d'Enfert Christophe, Dannaoui Eric, Bougnoux Marie-Elisabeth
Unité de Parasitologie-Mycologie, Service de Microbiologie, Faculté de Médecine, APHP, Hôpital Européen Georges Pompidou, Université Paris-Descartes, Paris, France.
Unité de Parasitologie-Mycologie, Service de Microbiologie, Faculté de Médecine, APHP, Hôpital Necker Enfants-Malades, Université Paris-Descartes, Paris, France.
Front Microbiol. 2020 Jun 16;11:1286. doi: 10.3389/fmicb.2020.01286. eCollection 2020.
When and isolates were tested for susceptibility to fluconazole and echinocandins using either EUCAST or Etest methods, differential patterns of growth were observed, independently of the methods used. For , a trailing phenomenon (incomplete growth inhibition at supra-MICs) was observed with fluconazole in 90% and 93.3% for EUCAST and Etest, respectively, but not with echinocandins (<7% for EUCAST and 0% for Etest). In contrast, for , a trailing phenomenon was very rarely observed with fluconazole (20% for EUCAST and 0% for Etest), while the opposite pattern was observed with echinocandins (>50% for EUCAST and >86% for Etest). This suggests that the pathways involved in the trailing effect might be different between these two related species. Furthermore, clinical microbiologists must be aware of these species-specific patterns for a reliable MIC determination.
当使用EUCAST或Etest方法检测菌株对氟康唑和棘白菌素的敏感性时，无论使用何种方法，均观察到不同的生长模式。对于[某种菌]，使用EUCAST和Etest方法时，分别有90%和93.3%的菌株在氟康唑检测中出现拖尾现象（在高于最低抑菌浓度时生长抑制不完全），但在棘白菌素检测中未出现（EUCAST检测中<7%，Etest检测中为0%）。相比之下，对于[另一种菌]，氟康唑检测中很少出现拖尾现象（EUCAST检测中为20%，Etest检测中为0%），而棘白菌素检测则出现相反模式（EUCAST检测中>50%，Etest检测中>86%）。这表明这两个相关菌种中涉及拖尾效应的途径可能不同。此外，临床微生物学家必须了解这些菌种特异性模式，以便进行可靠的最低抑菌浓度测定。
