Koc Feride, Tekeli Muhammet Yasin, Kanbur Murat, Karayigit Mehmet Önder, Liman Bilal Cem
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Erciyes University, Kayseri, Turkey.
Department of Pathology, Faculty of Veterinary Medicine, Cumhuriyet University, Sivas, Turkey.
J Food Biochem. 2020 Sep;44(9):e13359. doi: 10.1111/jfbc.13359. Epub 2020 Jul 2.
Chrysin (CR) is a flavone found in propolis and many plants. Lipopolysaccharide (LPS) is a component of the cell wall of gram-negative bacteria that causes sepsis. The purpose of this study was to investigate the effects of CR on LPS-induced sepsis in rats. LPS intraperitoneal and a single dose and CR were given orally for 10 days. Rats were sacrificed, blood samples were taken, liver, lung, and kidney tissues were dissected, homogenized, and histopathological analysis was carried out. When CR groups compared to sepsis group, CR significantly decreased the serum levels of aspartate transaminase (AST) and alanine aminotransferase (ALT), interleukin-1 beta (IL-1β), interleukin-10 (IL-10), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and levels of malondialdehyde (MDA) in tissues. CR also increased the levels of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in tissues. Histopathological findings were consistent with biochemical findings. Conclusion, CR could reduce the oxidative stress markers and cytokines in sepsis. PRACTICAL APPLICATIONS: Our approach is to determine the antioxidant and anti-inflammatory effects of chrysin, known as a flavolonoid, which are found in many plants and foods such as honey and propolis. In this study, experimental sepsis model was created using LPS. According to the results of the study, CR can attribute to the ameliorating of oxidative damage in tissues (lung, liver, and kidney) and it can suppress the sepsis-associated acute tissue injury via reduction of inflammation in rats. Even, CR can be used as a pharmacological agent in inflammatory diseases caused by other sources and in many cases causing oxidation.
白杨素（CR）是一种存在于蜂胶和许多植物中的黄酮。脂多糖（LPS）是革兰氏阴性菌细胞壁的一种成分，可导致败血症。本研究的目的是探讨CR对LPS诱导的大鼠败血症的影响。腹腔注射单剂量LPS，并口服CR，持续10天。处死大鼠，采集血样，解剖肝脏、肺和肾脏组织，进行匀浆，并进行组织病理学分析。与败血症组相比，CR组血清天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、白细胞介素-1β（IL-1β）、白细胞介素-10（IL-10）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）水平以及组织中丙二醛（MDA）水平显著降低。CR还提高了组织中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）的水平。组织病理学结果与生化结果一致。结论：CR可降低败血症中的氧化应激标志物和细胞因子。实际应用：我们的方法是确定白杨素的抗氧化和抗炎作用，白杨素是一种黄酮类化合物，存在于许多植物和食物中，如蜂蜜和蜂胶。在本研究中，使用LPS建立实验性败血症模型。根据研究结果，CR可改善组织（肺、肝和肾）的氧化损伤，并可通过减轻大鼠炎症来抑制败血症相关的急性组织损伤。甚至，CR可作为一种药物用于由其他来源引起的、在许多情况下会导致氧化的炎症性疾病。
