R&D Center, ACRO Biomedical Co., Ltd., Luzhu District, Kaohsiung City 82151, Taiwan.
Biotechniques. 2020 Sep;69(3):220-225. doi: 10.2144/btn-2020-0070. Epub 2020 Jul 3.
Traditional purification of atelocollagen involves a harsh extraction process with environment-polluting chemicals and costly and/or time-consuming procedures which include salting-out, alkali, acid and enzymatic treatment and ion-exchange chromatography. The atelocollagen market is growing exponentially, with demand in the skincare industry and for various medical applications. As a result, there is an urgent need for an eco-friendly production process with minimal manipulation. We developed a novel technique involving supercritical carbon dioxide extraction technology to remove the cells and noncollagenous substances from the porcine hide. Subsequent processes allow the production of several products, including decellularized dermal membrane, high-purity collagen particles and atelocollagen. The advantages of our process are its faster speed and lower environmental impact and its generation of multiple products, including high purity atelocollagen with complete removal of telopeptides.
传统的明胶原纤维的纯化过程涉及到使用环境污染化学物质的苛刻提取过程，以及包括盐析、碱处理、酸处理和酶处理以及离子交换层析等昂贵且/或耗时的步骤。随着对皮肤护理行业和各种医疗应用的需求不断增长，明胶原纤维市场呈指数级增长。因此，迫切需要一种环保的生产工艺，尽量减少操作。我们开发了一种新的技术，涉及超临界二氧化碳提取技术，以去除猪皮中的细胞和非胶原蛋白物质。随后的处理过程允许生产多种产品，包括脱细胞真皮膜、高纯度胶原蛋白颗粒和明胶原纤维。我们的工艺的优点是速度更快、对环境的影响更小，并且可以生成多种产品，包括完全去除端肽的高纯度明胶原纤维。
