Periasamy Srinivasan, Chen Yun-Ju, Hsu Dur-Zong, Hsieh Dar-Jen
R&D Center, ACRO Biomedical Co., Ltd, 2nd. Floor, No.57, Luke 2nd. Rd., Luzhu District, Kaohsiung City, 82151, Taiwan.
Department of Environmental and Occupational Health, College of Medicine, National Cheng Kung University, 138 Sheng-Li Rd., Tainan, 70428, Taiwan.
Bioresour Bioprocess. 2024 Feb 3;11(1):21. doi: 10.1186/s40643-024-00731-1.
Osteoarthritis (OA) of the knee is a common degenerative articular disorder and is one of the main causes of pain and functional disability. Cartilage damage is frequently linked to elevated osteoarthritis incidence. Supercritical carbon dioxide (scCO) decellularized cartilage graft produced from the porcine cartilage is an ideal candidate for cartilage tissue engineering. In the present study, we derived collagen type II (Col II) solution from the scCO decellularized porcine cartilage graft (dPCG) and compared its efficacy with hyaluronic acid (HA) in the surgical medial meniscectomy (MNX) induced post-traumatic osteoarthritis (PTOA) model. Dose-dependent attenuation of the OA (12.3 ± 0.8) progression was observed in the intra-articular administration of Col II solution (7.3 ± 1.2) which significantly decreased the MNX-induced OA symptoms similar to HA. The pain of the OA group (37.4 ± 2.7) was attenuated dose-dependently by Col II solution (45.9 ± 4.1) similar to HA (43.1 ± 3.5) as evaluated by a capacitance meter. Micro-CT depicted a dose-dependent attenuation of articular cartilage damage by the Col II solution similar to HA treatment. A significant (p < 0.001) dose-dependent elevation in the bone volume was also observed in Col II solution-treated OA animals. The protective competence of Col II solution on articular cartilage damage is due to its significant (p < 0.001) increase in the expression of type II collagen, aggrecan and SOX-9 similar to HA. To conclude, intra-articular administration of type II collagen solution and HA reestablished the injured cartilage and decreased osteoarthritis progression in the experimental PTOA model.
膝关节骨关节炎（OA）是一种常见的退行性关节疾病，是疼痛和功能障碍的主要原因之一。软骨损伤常与骨关节炎发病率升高有关。由猪软骨制备的超临界二氧化碳（scCO）脱细胞软骨移植物是软骨组织工程的理想候选材料。在本研究中，我们从scCO脱细胞猪软骨移植物（dPCG）中提取了II型胶原蛋白（Col II）溶液，并在手术内侧半月板切除术（MNX）诱导的创伤后骨关节炎（PTOA）模型中，将其疗效与透明质酸（HA）进行了比较。关节内注射Col II溶液（7.3±1.2）可观察到OA（12.3±0.8）进展的剂量依赖性减弱，这显著减轻了MNX诱导的OA症状，与HA相似。通过电容计评估，OA组（37.4±2.7）的疼痛被Col II溶液（45.9±4.1）剂量依赖性减轻，与HA（43.1±3.5）相似。显微CT显示，Col II溶液对关节软骨损伤的减轻作用与HA治疗相似，呈剂量依赖性。在Col II溶液治疗的OA动物中，还观察到骨体积显著（p<0.001）的剂量依赖性增加。Col II溶液对关节软骨损伤的保护能力归因于其与HA相似，II型胶原蛋白、聚集蛋白聚糖和SOX-9的表达显著（p<0.001）增加。总之，在实验性PTOA模型中，关节内注射II型胶原蛋白溶液和HA可修复受损软骨并减少骨关节炎进展。
