School of Allied Health Sciences, Aarupadi Veedu Medical College and Hospital Campus, Vinayaka Mission's Research Foundation (Deemed to be University), Puducherry, India.
Department of Microbiology, School of Biosciences, Periyar University, Salem, India.
Int J Phytoremediation. 2020;22(14):1462-1479. doi: 10.1080/15226514.2020.1782828. Epub 2020 Jul 2.
In present investigation carried out large-scale treatment of tannery effluent by the cultivation of microalgae, RDS02. The tannery effluent treatment revealed that significant reduction heavy metals were chromium-3.59, lead-2.85, nickel-1.9, cadmium-10.68, zinc-4.49, copper-0.95 and cobalt-1.86 mg/L on 15th day of treatment using RDS02. The microalgal biosorption capacity rate was Cr-88.66, Pb-75.87, Ni-87.61, Cd-60.44, Co-52.86, Zn-84.90 and Cu-54.39, and isotherm model emphasized that the higher value 0.99 by Langmuir and Freundlich kinetics model. The microalga utilized highest CO (90%) analyzed by CO biofixation and utilization kinetics, biomass (3.9 mg/mL), lipid (210 mg mL), carbohydrate (102.75 mg mL), biodiesel (4.9 mL g) and bioethanol (4.1 mL g). The microalgal-lipid content was analyzed through Nile red staining. Gas chromatography mass spectrometric (GCMS) analysis confirmed that the presence of a biodiesel and major fatty acid methyl ester (FAME) profiling ., tridecanoic acid methyl ester, pentadecanoic acid methyl ester, octadecanoic acid methyl ester, myristic acid methyl ester, palmitic acid methyl ester and oleic acid methyl ester. Fourier transform infrared (FTIR) analysis confirmed that the presence of a functional groups ., phenols, alcohols, alkynes, carboxylic acids, ketones, carbonyl and ester groups. The bioethanol production was confirmed by high-performance liquid chromatography (HPLC) analyze.
在本次研究中，通过培养微藻 RDS02 对制革废水进行了大规模处理。制革废水处理结果表明，在使用 RDS02 处理 15 天后，重金属铬、铅、镍、镉、锌、铜和钴的去除率分别为 3.59、2.85、1.9、10.68、4.49、0.95 和 1.86mg/L。微藻的生物吸附容量分别为铬 88.66、铅 75.87、镍 87.61、镉 60.44、钴 52.86、锌 84.90 和铜 54.39，等温模型强调，Langmuir 和 Freundlich 动力学模型的更高值为 0.99。微藻通过 CO 生物固定和利用动力学分析，利用最高的 CO（90%），生物量（3.9mg/mL）、脂质（210mg/mL）、碳水化合物（102.75mg/mL）、生物柴油（4.9mL/g）和生物乙醇（4.1mL/g）。通过尼罗红染色分析微藻的脂质含量。气相色谱-质谱联用（GCMS）分析证实了生物柴油的存在和主要脂肪酸甲酯（FAME）分析。存在肉豆蔻酸甲酯、十五烷酸甲酯、十八烷酸甲酯、肉豆蔻酸甲酯、棕榈酸甲酯和油酸甲酯。傅里叶变换红外（FTIR）分析证实了存在功能基团。存在酚类、醇类、炔烃、羧酸、酮类、羰基和酯类。通过高效液相色谱（HPLC）分析证实了生物乙醇的生产。
