Nakai S, Mizuno K, Kaneta M, Hirai Y
Laboratories of Cellular Technology, Otsuka Parmaceutical Co., Ltd., Japan.
Biochem Biophys Res Commun. 1988 Aug 15;154(3):1189-96. doi: 10.1016/0006-291x(88)90266-5.
Interleukin-1 (IL-1) exhibits multiple biological properties on various tissues by modulating immunologic, inflammatory, metabolic, and neurologic functions. Considerable attention has focussed on the measurement of IL-1 activity. We reported a simple, sensitive, and specific bioassay for IL-1 using human melanoma A375 subclone which is highly sensitive for the cell growth inhibitory activity of IL-1. This bioassay method is allows detection of as low as 10pg of IL-1 beta/ml or 30pg of IL-1 alpha/ml. Since this A375 subclone cell dose not respond to prostaglandin E2 plant lectins, lipopolysaccharide, and cytokines such as interleukin-2, interleukin-6, tumor necrosis factor, interferon or colony-stimulating factor, it is an extremely useful and rapid method for the measurement of IL-1 activity in a variety of experimental and clinical conditions. The assay method was used in the presence of antisera to IL-1 beta to discriminate two species of IL-1, IL-1 alpha and IL-1 beta, produced in human peripheral mononuclear cells.
白细胞介素-1（IL-1）通过调节免疫、炎症、代谢和神经功能，在各种组织上表现出多种生物学特性。人们对IL-1活性的测量给予了相当多的关注。我们报道了一种使用人黑色素瘤A375亚克隆的简单、灵敏且特异的IL-1生物测定法，该亚克隆对IL-1的细胞生长抑制活性高度敏感。这种生物测定方法能够检测低至10pg/ml的IL-1β或30pg/ml的IL-1α。由于该A375亚克隆细胞对前列腺素E2、植物凝集素、脂多糖以及白细胞介素-2、白细胞介素-6、肿瘤坏死因子、干扰素或集落刺激因子等细胞因子无反应，因此它是在各种实验和临床条件下测量IL-1活性的一种极其有用且快速的方法。该测定方法在存在抗IL-1β血清的情况下用于区分人外周血单个核细胞产生的两种IL-1，即IL-1α和IL-1β。
