Yamaguchi N, Yamashita Y, Ikeda D, Koga T
Department of Preventive Dentistry, Kyushu University Faculty of Dentistry, Fukuoka, Japan.
Infect Immun. 1996 Jul;64(7):2563-70. doi: 10.1128/iai.64.7.2563-2570.1996.
Serotype b-specific polysaccharide antigen (SPA) was extracted from whole cells of Actinobacillus actinomycetemcomitans Y4 by autoclaving and purified by chromatography on DEAE-Sephadex A-25 and Sephacryl S-300. SPA induced the release of monocyte and leukocyte chemotactic factors by human monocytes. Polymyxin B had almost no effect on the release of monocyte chemotactic factor, but a monoclonal antibody against SPA markedly inhibited it. Human monocytes stimulated with SPA exhibited the increased mRNA expression of monocyte chemoattractant protein 1 (MCP-1) and a neutrophil chemotactic factor, interleukin-8 (IL-8). On the other hand, SPA induced the release of IL-1, IL-6, and tumor necrosis factor (TNF) and enhanced the expression of IL-1alpha, IL-1beta, IL-6, and TNF alpha (TNF-alpha) mRNAs. Human monocytes expressed MCP-1 and IL-8 mRNAs when stimulated by human recombinant IL-1alpha, I1-1beta, IL-6, and TNF-alpha, suggesting that these inflammatory cytokines induced by SPA might participate in the production of chemotactic factors in human monocytes.
b型特异性多糖抗原（SPA）通过高压灭菌从伴放线放线杆菌Y4的全细胞中提取，并通过在DEAE-葡聚糖A-25和Sephacryl S-300上进行色谱法纯化。SPA可诱导人单核细胞释放单核细胞和白细胞趋化因子。多粘菌素B对单核细胞趋化因子的释放几乎没有影响，但抗SPA单克隆抗体可显著抑制其释放。用SPA刺激的人单核细胞显示单核细胞趋化蛋白1（MCP-1）和中性粒细胞趋化因子白细胞介素-8（IL-8）的mRNA表达增加。另一方面，SPA可诱导IL-1、IL-6和肿瘤坏死因子（TNF）的释放，并增强IL-1α、IL-1β、IL-6和TNFα（TNF-α）mRNA的表达。当受到人重组IL-1α、I1-1β、IL-6和TNF-α刺激时，人单核细胞表达MCP-1和IL-8 mRNA，这表明SPA诱导的这些炎性细胞因子可能参与人单核细胞中趋化因子的产生。
