Jeong H D, Teale J M
Department of Microbiology, University of Texas Health Science Center, San Antonio 78284.
J Exp Med. 1988 Aug 1;168(2):589-603. doi: 10.1084/jem.168.2.589.
The functional B cell repertoire in BALB/c mice was assessed at various stages in ontogeny. This was done by analyzing VH gene family expression using the sensitive technique of in situ hybridization. The B cell repertoire was probed with the mitogen, LPS, and the antigen DNP. DNP was chosen because B cells responsive to this hapten appear very early in ontogeny. The APCs that developed after stimulation with LPS or DNP were analyzed for VH gene expression by in situ hybridization of individual cells using radiolabeled VH gene family probes. The results indicated that VH gene expression in fetal B cells after stimulation was distinct from adult B cells in that there was a biased expression of D proximal families. The results indicated that this bias was associated with developmental age and not a given differentiation stage in the B cell lineage. In addition, stimulation of fetal B cells with DNP resulted in a large increase in expression of member(s) of VH 36-60, suggesting that the early appearance of DNP-responsive B cells is not strictly correlated with preferential rearrangement of D proximal families, VH 7183 and VH Q52. However, the results suggested that a large proportion of pre-B cells that preferentially rearrange D proximal families early in ontogeny become part of the functional developing repertoire.
在个体发育的不同阶段评估了BALB/c小鼠的功能性B细胞库。这是通过使用原位杂交的敏感技术分析VH基因家族表达来完成的。用丝裂原LPS和抗原DNP探测B细胞库。选择DNP是因为对这种半抗原产生反应的B细胞在个体发育中出现得非常早。用放射性标记的VH基因家族探针通过单个细胞的原位杂交分析用LPS或DNP刺激后产生的抗原呈递细胞（APC）的VH基因表达。结果表明，刺激后胎儿B细胞中的VH基因表达与成年B细胞不同，在于D近端家族存在偏向性表达。结果表明，这种偏向性与发育年龄相关，而不是B细胞谱系中的特定分化阶段。此外，用DNP刺激胎儿B细胞导致VH 36-60成员的表达大幅增加，这表明对DNP产生反应的B细胞的早期出现与D近端家族VH 7183和VH Q52的优先重排并不严格相关。然而，结果表明，在个体发育早期优先重排D近端家族的很大一部分前B细胞成为功能性发育库的一部分。
