Edström A, Kanje M
Department of Zoophysiology, University of Lund, Sweden.
Neurosci Lett. 1988 Jul 19;90(1-2):113-8. doi: 10.1016/0304-3940(88)90796-3.
The sensory axons of the adult frog sciatic nerve were found to regenerate from a local test crush in vitro, providing the nerve was subjected to a conditioning lesion in vivo 10-17 days earlier. If the latter was applied distal to the test lesion, the regeneration rate was 1.1 mm/day or only somewhat slower than in vivo. The elongation rate in vitro was maintained at an unchanged rate for at least 9 days. With the use of a two-chambered culture system we could show that the growth was dependent on local protein synthesis. If the conditioning and test lesions were both applied at the same site, the outgrowth distance after 5 days was significantly longer than when the lesions were separated and also longer than the regeneration distance 5 days after a single test lesion in vivo. The results show that the growth process is influenced by local factors and can be maintained in vitro in the absence of blood-borne signals. The present system opens unique possibilities for exploring the role of potential growth factors and other conditions involved in axonal regeneration in an adult vertebrate peripheral nerve.
成年青蛙坐骨神经的感觉轴突在体外局部测试挤压后被发现能够再生，前提是该神经在10 - 17天前在体内接受了预处理损伤。如果后者施加在测试损伤的远端，再生速率为1.1毫米/天，或者仅比在体内稍慢。体外伸长速率至少9天保持不变。通过使用双室培养系统，我们可以证明生长依赖于局部蛋白质合成。如果预处理损伤和测试损伤都施加在同一部位，5天后的生长距离显著长于损伤分开时的情况，也长于体内单次测试损伤5天后的再生距离。结果表明，生长过程受局部因素影响，并且在没有血源性信号的情况下可以在体外维持。本系统为探索潜在生长因子和其他参与成年脊椎动物外周神经轴突再生的条件的作用提供了独特的可能性。
