Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark.
Novo Nordisk Foundation Center for Stem Cell Biology (DanStem), Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark.
J Histochem Cytochem. 2020 Aug;68(8):561-570. doi: 10.1369/0022155420940220. Epub 2020 Jul 3.
Cells of the human breast gland express an array of keratins, of which some are used for characterizing both normal and neoplastic breast tissue. However, the expression pattern of certain keratins has yet to be detailed. Here, the expression of a differentiation marker of epidermal epithelium, keratin 10 (K10), was investigated in the human breast gland. While in normal breast tissue generally less than 1% of luminal epithelial cells expressed K10, in women >30 years of age glandular structures with K10-positive (K10) cells were found at higher frequency than in younger women. K10 cells belong to a mature luminal compartment as they were negative for cKIT, positive for Ks20.8, and mostly non-cycling. In breast cancer, around 16% of primary breast carcinomas tested were positive for K10 by immunohistochemistry. Interestingly, K10 tumor cells generally exhibit features of differentiation similar to their normal counterparts. Although this suggests that K10 is a marker of tumor differentiation, data based on gene expression analysis imply that high levels of K10 dictate a worse outcome for breast cancer patients. These findings can form the basis of future studies that should unravel which role K10 may play as a marker of breast cancer.
人类乳腺细胞表达一系列角蛋白，其中一些用于描述正常和肿瘤性乳腺组织。然而，某些角蛋白的表达模式尚未详细描述。在此，研究了表皮上皮分化标志物角蛋白 10（K10）在人类乳腺中的表达。在正常乳腺组织中，仅有不到 1%的腔上皮细胞表达 K10，但在>30 岁的女性中，发现具有 K10 阳性（K10）细胞的腺结构比年轻女性更常见。K10 细胞属于成熟的腔上皮部分，因为它们对 cKIT 呈阴性，对 Ks20.8 呈阳性，且大多处于非循环状态。在乳腺癌中，约 16%的原发性乳腺癌经免疫组化检测呈 K10 阳性。有趣的是，K10 肿瘤细胞通常表现出与其正常对应物相似的分化特征。尽管这表明 K10 是肿瘤分化的标志物，但基于基因表达分析的数据表明，高水平的 K10 预示着乳腺癌患者的预后更差。这些发现可以为未来的研究奠定基础，这些研究应阐明 K10 作为乳腺癌标志物可能发挥的作用。
