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p63与HDAC1的共表达模式：一种揭示毛囊间表皮干细胞的潜在方法。

The Co-Expression Pattern of p63 and HDAC1: A Potential Way to Disclose Stem Cells in Interfollicular Epidermis.

作者信息

Shin Jung-Won, Choi Hye-Ryung, Nam Kyung-Mi, Lee Hyun-Sun, Kim Sung-Ae, Joe Hyun-Jae, Kazumi Toyama, Park Kyoung-Chan

机构信息

Department of Dermatology, Seoul National University Bundang Hospital, 166 Gumi-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-707, Korea.

Department of Dermatology, Keimyung University School of Medicine, 56 Dalseong-Ro, Jung-Gu, Daegu 41931, Korea.

出版信息

Int J Mol Sci. 2017 Jun 26;18(7):1360. doi: 10.3390/ijms18071360.

DOI:10.3390/ijms18071360
PMID:28672879
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5535853/
Abstract

Stem cell markers of interfollicular epidermis (IEF) have not been established thus far. The aim of this study is to suggest a new way to disclose IFE-stem cells by combining the expression of histone deacetylases (HDAC) 1 and p63. Immunohistochemical staining of HDAC1 and p63 was performed in six normal human samples. Moreover, a skin equivalent (SE) model was treated with suberoylanilohydroxamic acid (SAHA, an HDAC inhibitor) to elucidate the role of HDAC1. Finally, rapidly adhering (RA) keratinocytes to a type IV collagen, which have been identified to represent epidermal stem cells, were subjected to Western blot analysis with antibodies against HDAC1. In normal samples, there was a minor subpopulation comprised of p63-positive and HDAC1-negative cells in the basal layers. The proportion of this subpopulation was decreased with age. In the SE model, SAHA treatment increased the epidermal thickness and number of p63-positive cells in a dose dependent manner. After SAHA treatment, the expression of differentiation markers was decreased, while that of basement membrane markers was increased. In a Western blot analysis, HDAC1 was not expressed in RA cells. In conclusion, the combination of p63-positive and HDAC1-negative expressions can be a potential new way for distinguishing epidermal stem cells.

摘要

迄今为止，尚未确定毛囊间表皮（IEF）的干细胞标志物。本研究的目的是提出一种通过结合组蛋白去乙酰化酶（HDAC）1和p63的表达来揭示IEF干细胞的新方法。对六个正常人体样本进行了HDAC1和p63的免疫组织化学染色。此外，用辛二酰苯胺异羟肟酸（SAHA，一种HDAC抑制剂）处理皮肤等效物（SE）模型，以阐明HDAC1的作用。最后，对已被确定代表表皮干细胞的、能快速黏附于IV型胶原的（RA）角质形成细胞，用抗HDAC1抗体进行蛋白质印迹分析。在正常样本中，基底层有一个由p63阳性和HDAC1阴性细胞组成的较小亚群。该亚群的比例随年龄增长而降低。在SE模型中，SAHA处理以剂量依赖方式增加了表皮厚度和p63阳性细胞的数量。SAHA处理后，分化标志物的表达降低，而基底膜标志物的表达增加。在蛋白质印迹分析中，RA细胞中未表达HDAC1。总之，p63阳性和HDAC1阴性表达的组合可能是区分表皮干细胞的一种潜在新方法。

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