用于细菌细胞大规模紫外线照射的连续流动系统。
Continuous-flow system for large-scale ultraviolet irradiation of bacterial cells.
作者信息
Joshi J G, Swenson P A
出版信息
Appl Environ Microbiol. 1977 Apr;33(4):915-7. doi: 10.1128/aem.33.4.915-917.1977.
Abstract
A quartz-flow-cell system for irradiation of large volumes of Escherichia coli cultures with ultraviolet light is described. With this system kilogram quantities of irradiated cells can be obtained for biochemical studies. Changes in respiration and in specific activities of superoxide dismutase and catalase, after an ultraviolet treatment that reduced viability of culture samples to 0.2%, were in good agreement with those for cultures irradiated (52J/m2) by a conventional small-scale method to produce the same reduction in viability.
摘要
描述了一种用于用紫外线照射大量大肠杆菌培养物的石英流动池系统。使用该系统可获得数千克经照射的细胞用于生化研究。在将培养样品的活力降低至0.2%的紫外线处理后，呼吸作用以及超氧化物歧化酶和过氧化氢酶的比活性变化，与通过传统小规模方法（52J/m²）照射培养物以产生相同活力降低的情况高度一致。
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本文引用的文献
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A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase.一种用于测量过氧化氢被过氧化氢酶分解的分光光度法。
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Evidence for the control of respiration by DNA in ultraviolet-irradiated Escherichia coli B-r cells.紫外线照射的大肠杆菌B-r细胞中DNA对呼吸作用控制的证据。
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Evidence relating cessation of respiration, cell envelope changes, and death in ultraviolet-irradiated Escherichia coli B-r cells.关于紫外线照射的大肠杆菌B-r细胞中呼吸停止、细胞包膜变化和死亡的相关证据。
J Bacteriol. 1974 Feb;117(2):551-9. doi: 10.1128/jb.117.2.551-559.1974.
4
Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase.超氧阴离子自由基在邻苯三酚自氧化中的作用及超氧化物歧化酶的简易测定法
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Superoxide dismutases.超氧化物歧化酶
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Centrifugal separation of irradiated cultures of Escherichia coli cells into viable and nonviable populations.将经辐照的大肠杆菌细胞培养物离心分离成活细胞群体和非活细胞群体。
J Bacteriol. 1976 May;126(2):977-84. doi: 10.1128/jb.126.2.977-984.1976.
7
Delayed ultraviolet light-induced cessation of respiration by inadequate aeration of Escherichia coli.因大肠杆菌通气不足导致的紫外线诱导呼吸延迟停止。
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