Abdallah J M, Kukuruga M, Nakeff A, Prasad A S
Department of Medicine, Wayne State University School of Medicine, Harper-Grace Hospital, Detroit, Michigan.
Am J Hematol. 1988 Aug;28(4):279-81. doi: 10.1002/ajh.2830280414.
T lymphocytes from normal human controls and sickle cell disease (SCD) patients were isolated from peripheral blood and cultured for 72 hours following addition of phytohemagglutinin. The ratio for the fraction of cells in DNA synthesis (S phase) over the fraction in G2 phase (S/G2) was significantly higher in SCD patients in comparison to the controls (mean +/- SD) (4.01 +/- 0.78 vs. 2.78 +/- 0.76, P less than 0.02). Following in vivo zinc supplementation to two subjects, the S/G2 ratio was normalized. We conclude that the distribution of T lymphocytes in cell cycle is altered in SCD patients and that this effect may be zinc-dependent.
从正常人类对照者和镰状细胞病（SCD）患者的外周血中分离出T淋巴细胞，并在添加植物血凝素后培养72小时。与对照组相比，SCD患者中处于DNA合成期（S期）的细胞分数与处于G2期的细胞分数之比（S/G2）显著更高（平均值±标准差）（4.01±0.78对2.78±0.76，P<0.02）。对两名受试者进行体内补锌后，S/G2比值恢复正常。我们得出结论，SCD患者T淋巴细胞在细胞周期中的分布发生了改变，且这种影响可能依赖于锌。
