• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

hUMSC 移植通过 AMPK/mTOR 信号通路抑制颗粒细胞-间质细胞自噬来恢复 POI 大鼠的卵巢功能。

hUMSC transplantation restores ovarian function in POI rats by inhibiting autophagy of theca-interstitial cells via the AMPK/mTOR signaling pathway.

机构信息

College of Basic Medicine & Institute of Reproductive Diseases, Binzhou Medical University, Yantai, 264003, Shandong, China.

College of Basic Medicine, Binzhou Medical University, Yantai, 264003, Shandong, China.

出版信息

Stem Cell Res Ther. 2020 Jul 3;11(1):268. doi: 10.1186/s13287-020-01784-7.

DOI:10.1186/s13287-020-01784-7
PMID:32620136
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7333437/
Abstract

BACKGROUND

Previous studies of primary ovarian insufficiency (POI) have focused on granulosa cells (GCs) and ignored the role of theca-interstitial cells (TICs). This study aims to explore the mechanism of the protective effects of human umbilical cord-derived mesenchymal stem cells (hUMSCs) on ovarian function in POI rats by regulating autophagy of TICs.

METHODS

The POI model was established in rats treated with cisplatin (CDDP). The hUMSCs were transplanted into POI rats by tail vein. Enzyme-linked immunosorbent assay (ELISA) analysis, hematoxylin and eosin (HE) staining, and immunohistochemistry were used to measure the protective effects of hUMSCs. The molecular mechanisms of injury and repairment of TICs were assessed by immunofluorescence, transmission electron microscope (TEM), flow cytometry (FCM), western blot, and quantitative real-time polymerase chain reaction (qRT-PCR).

RESULTS

In vivo, hUMSC transplantation restored the ovarian function and alleviated the apoptosis of TICs in POI rats. In vitro, hUMSCs reduced the autophagy levels of TICs by reducing oxidative stress and regulating AMPK/mTOR signaling pathway, thereby alleviating the apoptosis of TICs.

CONCLUSION

This study indicates that hUMSCs protected ovarian function in POI by regulating autophagy signaling pathway AMPK/mTOR.

摘要

背景

先前关于原发性卵巢功能不全 (POI) 的研究主要集中在颗粒细胞 (GCs) 上，而忽略了卵丘间质细胞 (TICs) 的作用。本研究旨在通过调节 TIC 自噬来探讨人脐带间充质干细胞 (hUMSC) 对 POI 大鼠卵巢功能的保护作用机制。

方法

采用顺铂 (CDDP) 处理建立 POI 大鼠模型。通过尾静脉将 hUMSC 移植到 POI 大鼠中。采用酶联免疫吸附试验 (ELISA) 分析、苏木精和伊红 (HE) 染色和免疫组织化学检测 hUMSC 的保护作用。通过免疫荧光、透射电子显微镜 (TEM)、流式细胞术 (FCM)、Western blot 和实时定量聚合酶链反应 (qRT-PCR) 评估 TIC 损伤和修复的分子机制。

结果

体内，hUMSC 移植恢复了 POI 大鼠的卵巢功能并减轻了 TIC 的凋亡。体外，hUMSCs 通过减少氧化应激和调节 AMPK/mTOR 信号通路降低了 TIC 的自噬水平，从而减轻了 TIC 的凋亡。

结论

本研究表明，hUMSC 通过调节 AMPK/mTOR 自噬信号通路保护 POI 中的卵巢功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02eb/7333437/16250caa2069/13287_2020_1784_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02eb/7333437/974922063e8e/13287_2020_1784_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02eb/7333437/e3d4c68eda0d/13287_2020_1784_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02eb/7333437/b132acf725c0/13287_2020_1784_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02eb/7333437/6cb2a5d8896a/13287_2020_1784_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02eb/7333437/a1e2d598031d/13287_2020_1784_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02eb/7333437/73088ddef31b/13287_2020_1784_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02eb/7333437/16250caa2069/13287_2020_1784_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02eb/7333437/974922063e8e/13287_2020_1784_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02eb/7333437/e3d4c68eda0d/13287_2020_1784_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02eb/7333437/b132acf725c0/13287_2020_1784_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02eb/7333437/6cb2a5d8896a/13287_2020_1784_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02eb/7333437/a1e2d598031d/13287_2020_1784_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02eb/7333437/73088ddef31b/13287_2020_1784_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02eb/7333437/16250caa2069/13287_2020_1784_Fig7_HTML.jpg

相似文献

1
hUMSC transplantation restores ovarian function in POI rats by inhibiting autophagy of theca-interstitial cells via the AMPK/mTOR signaling pathway.hUMSC 移植通过 AMPK/mTOR 信号通路抑制颗粒细胞-间质细胞自噬来恢复 POI 大鼠的卵巢功能。
Stem Cell Res Ther. 2020 Jul 3;11(1):268. doi: 10.1186/s13287-020-01784-7.
2
hUMSCs Transplantation Regulates AMPK/NR4A1 Signaling Axis to Inhibit Ovarian Fibrosis in POI Rats.人脐带间充质干细胞移植通过调控 AMPK/NR4A1 信号轴抑制 POI 大鼠卵巢纤维化。
Stem Cell Rev Rep. 2023 Jul;19(5):1449-1465. doi: 10.1007/s12015-022-10469-y. Epub 2022 Oct 29.
3
hUMSCs regulate the differentiation of ovarian stromal cells via TGF-β/Smad3 signaling pathway to inhibit ovarian fibrosis to repair ovarian function in POI rats.hUMSCs 通过 TGF-β/Smad3 信号通路调节卵巢基质细胞的分化，以抑制卵巢纤维化，从而修复 POI 大鼠的卵巢功能。
Stem Cell Res Ther. 2020 Sep 7;11(1):386. doi: 10.1186/s13287-020-01904-3.
4
hUCMSCs reduce theca interstitial cells apoptosis and restore ovarian function in premature ovarian insufficiency rats through regulating NR4A1-mediated mitochondrial mechanisms.hUCMSCs 通过调节 NR4A1 介导的线粒体机制减少早发性卵巢功能不全大鼠卵巢间质细胞凋亡并恢复卵巢功能。
Reprod Biol Endocrinol. 2022 Aug 19;20(1):125. doi: 10.1186/s12958-022-00992-5.
5
hUMSCs restore ovarian function in POI mice by regulating GSK3β-mediated mitochondrial dynamic imbalances in theca cells.hUMSCs 通过调节颗粒细胞中 GSK3β 介导的线粒体动态失衡来恢复 POI 小鼠的卵巢功能。
Sci Rep. 2024 Aug 16;14(1):19008. doi: 10.1038/s41598-024-69381-9.
6
VX-765 enhances autophagy of human umbilical cord mesenchymal stem cells against stroke-induced apoptosis and inflammatory responses via AMPK/mTOR signaling pathway.VX-765 通过 AMPK/mTOR 信号通路增强人脐带间充质干细胞的自噬，对抗卒中诱导的细胞凋亡和炎症反应。
CNS Neurosci Ther. 2020 Sep;26(9):952-961. doi: 10.1111/cns.13400. Epub 2020 May 27.
7
Bu-Shen-Ning-Xin decoction alleviates premature ovarian insufficiency (POI) by regulating autophagy of granule cells through activating PI3K/AKT/mTOR pathway.补肾宁心汤通过激活 PI3K/AKT/mTOR 通路调节颗粒细胞自噬来缓解卵巢早衰（POI）。
Gynecol Endocrinol. 2022 Sep;38(9):754-764. doi: 10.1080/09513590.2022.2112941. Epub 2022 Aug 21.
8
Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) alleviate excessive autophagy of ovarian granular cells through VEGFA/PI3K/AKT/mTOR pathway in premature ovarian failure rat model.人脐带间充质干细胞（hUC-MSCs）通过 VEGFA/PI3K/AKT/mTOR 通路减轻卵巢早衰大鼠模型中卵巢颗粒细胞的过度自噬。
J Ovarian Res. 2023 Sep 30;16(1):198. doi: 10.1186/s13048-023-01278-z.
9
hUMSC transplantation restores follicle development in ovary damaged mice via re-establish extracellular matrix (ECM) components.hUMSC 移植通过重新建立细胞外基质（ECM）成分来恢复受损卵巢中卵泡的发育。
J Ovarian Res. 2023 Aug 24;16(1):172. doi: 10.1186/s13048-023-01217-y.
10
Exosomal miRNA-17-5p derived from human umbilical cord mesenchymal stem cells improves ovarian function in premature ovarian insufficiency by regulating SIRT7.人脐带间充质干细胞来源的外泌体 miR-17-5p 通过调控 SIRT7 改善卵巢早衰患者卵巢功能
Stem Cells. 2020 Sep;38(9):1137-1148. doi: 10.1002/stem.3204. Epub 2020 May 29.

引用本文的文献

1
Optimal transplantation strategies for stem cell treatment in premature ovarian failure: a network meta-analysis.干细胞治疗早发性卵巢功能不全的最佳移植策略：一项网状Meta分析
Stem Cell Res Ther. 2025 Aug 27;16(1):461. doi: 10.1186/s13287-025-04569-y.
2
Investigation of the Efficacy of Umbilical Cord Mesenchymal Stem Cell and Melatonin Treatment in Premature Ovarian Failure Model.脐带间充质干细胞与褪黑素治疗对卵巢早衰模型疗效的研究
Reprod Sci. 2025 Aug 14. doi: 10.1007/s43032-025-01942-3.
3
Precision medicine in premature ovarian insufficiency: a focus on the precision therapeutic strategies for mesenchymal stem cells.

本文引用的文献

1
The effects of human umbilical cord-derived mesenchymal stem cell transplantation on endometrial receptivity are associated with Th1/Th2 balance change and uNK cell expression of uterine in autoimmune premature ovarian failure mice.人脐带间充质干细胞移植对子宫内膜容受性的影响与 Th1/Th2 平衡改变及 uNK 细胞在自身免疫性卵巢早衰小鼠子宫中的表达有关。
Stem Cell Res Ther. 2019 Jul 22;10(1):214. doi: 10.1186/s13287-019-1313-y.
2
Resveratrol alleviates chemotherapy-induced oogonial stem cell apoptosis and ovarian aging in mice.白藜芦醇可减轻化疗诱导的小鼠卵原干细胞凋亡和卵巢衰老。
Aging (Albany NY). 2019 Feb 14;11(3):1030-1044. doi: 10.18632/aging.101808.
3
精准医学在卵巢早衰中的应用：聚焦间充质干细胞的精准治疗策略
Stem Cell Res Ther. 2025 Jul 16;16(1):381. doi: 10.1186/s13287-025-04512-1.
4
The risk factors, pathogenesis and treatment of premature ovarian insufficiency.卵巢早衰的危险因素、发病机制及治疗
J Ovarian Res. 2025 Jun 18;18(1):134. doi: 10.1186/s13048-025-01714-2.
5
hUMSC-derived exosomes alleviate follicular interstitial cell autophagy by let-7a-5p/AMPK/mTOR axis in POI rats.人脐带间充质干细胞来源的外泌体通过let-7a-5p/AMPK/mTOR轴减轻卵巢早衰大鼠卵泡间质细胞的自噬。
Stem Cell Res Ther. 2025 Jun 7;16(1):291. doi: 10.1186/s13287-025-04396-1.
6
Mesenchymal stem cells enchanced by salidroside to inhibit ferroptosis and improve premature ovarian insufficiency via Keap1/Nrf2/GPX4 signaling.红景天苷增强间充质干细胞通过Keap1/Nrf2/GPX4信号通路抑制铁死亡并改善卵巢早衰。
Redox Rep. 2025 Dec;30(1):2455914. doi: 10.1080/13510002.2025.2455914. Epub 2025 Jan 28.
7
Intraovarian injection of 3D-MSC-EVs-ECM gel significantly improved rat ovarian function after chemotherapy.三维 MSC-EVs-ECM 凝胶经卵巢内注射后，可显著改善化疗后大鼠的卵巢功能。
Reprod Biol Endocrinol. 2024 Oct 16;22(1):125. doi: 10.1186/s12958-024-01299-3.
8
hUMSCs restore ovarian function in POI mice by regulating GSK3β-mediated mitochondrial dynamic imbalances in theca cells.hUMSCs 通过调节颗粒细胞中 GSK3β 介导的线粒体动态失衡来恢复 POI 小鼠的卵巢功能。
Sci Rep. 2024 Aug 16;14(1):19008. doi: 10.1038/s41598-024-69381-9.
9
Deletion of Fbxw7 in oocytes causes follicle loss and premature ovarian insufficiency in mice.Fbxw7 缺失导致卵母细胞中卵泡耗竭和小鼠卵巢早衰。
J Cell Mol Med. 2024 Jun;28(12):e18487. doi: 10.1111/jcmm.18487.
10
3D hUC-MSC spheroids exhibit superior resistance to autophagy and apoptosis of granulosa cells in POF rat model.3D hUC-MSC 球体表现出对 POF 大鼠模型中颗粒细胞自噬和凋亡的更高抗性。
Reproduction. 2024 Jul 13;168(2). doi: 10.1530/REP-23-0496. Print 2024 Aug 1.
Regulation of the innate immune system by autophagy: monocytes, macrophages, dendritic cells and antigen presentation.
自噬对固有免疫系统的调控：单核细胞、巨噬细胞、树突状细胞和抗原呈递。
Cell Death Differ. 2019 Mar;26(4):715-727. doi: 10.1038/s41418-019-0297-6. Epub 2019 Feb 8.
4
Berberine attenuates apoptosis in rat retinal Müller cells stimulated with high glucose via enhancing autophagy and the AMPK/mTOR signaling.小檗碱通过增强自噬和 AMPK/mTOR 信号通路减轻高糖诱导的大鼠视网膜 Müller 细胞凋亡。
Biomed Pharmacother. 2018 Dec;108:1201-1207. doi: 10.1016/j.biopha.2018.09.140. Epub 2018 Oct 2.
5
Alleviation of endoplasmic reticulum stress protects against cisplatin-induced ovarian damage.减轻内质网应激可预防顺铂诱导的卵巢损伤。
Reprod Biol Endocrinol. 2018 Sep 3;16(1):85. doi: 10.1186/s12958-018-0404-4.
6
Lycopene ameliorates oxidative stress in the aging chicken ovary via activation of Nrf2/HO-1 pathway.番茄红素通过激活Nrf2/HO-1通路改善衰老鸡卵巢中的氧化应激。
Aging (Albany NY). 2018 Aug 16;10(8):2016-2036. doi: 10.18632/aging.101526.
7
Molecular Genetics of Premature Ovarian Insufficiency.原发性卵巢功能不全的分子遗传学
Trends Endocrinol Metab. 2018 Nov;29(11):795-807. doi: 10.1016/j.tem.2018.07.002. Epub 2018 Aug 2.
8
Involvement of mitophagy in cisplatin-induced cell death regulation.线粒体自噬在顺铂诱导的细胞死亡调控中的作用。
Biol Chem. 2019 Jan 28;400(2):161-170. doi: 10.1515/hsz-2018-0210.
9
Transplanted Mesenchymal Stem Cells Reduce Autophagic Flux in Infarcted Hearts via the Exosomal Transfer of miR-125b.移植间充质干细胞通过外泌体转移 miR-125b 减少梗死心脏中的自噬通量。
Circ Res. 2018 Aug 17;123(5):564-578. doi: 10.1161/CIRCRESAHA.118.312758.
10
Reproductive, Physiological, and Molecular Outcomes in Female Mice Deficient in Dhh and Ihh.Dhh 和 Ihh 基因缺失的雌性小鼠的生殖、生理和分子结果。
Endocrinology. 2018 Jul 1;159(7):2563-2575. doi: 10.1210/en.2018-00095.