Department of Molecular Medicine, School of Medicine and Dentistry|, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
School of Medical and Health Science, Edith Cowan University, Joondalup, Australia.
Virol J. 2020 Jul 3;17(1):91. doi: 10.1186/s12985-020-01376-0.
SLC10A1 gene codes NTCP, a receptor through which the hepatitis B virus (HBV) gets access into hepatocytes - a stage of the viral cycle necessary for replication. Polymorphism variants of SLC10A1 play roles in HBV infection, viral clearance, treatment outcome, and complications, in diverse ethnic groups and countries. However, no such study has been conducted in the Ghanaian population, a country with HBV endemicity. Therefore, an exploratory study was conducted to investigate the presence of three (3) single nucleotide polymorphisms (SNPs) in the SLC10A1 gene (rs2296651, rs61745930, and rs4646287) and assessed the risk of HBV infection among the Ghanaian population.
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to determine the presence of the SNPs among 292 participants comprising 146 HBV infected persons as case-subjects and 146 HBV non-infected persons as control-subjects.
The minor allele frequency (T) of rs2296651 was present in a significantly high proportion of cases compared with the control group (11.6% vs. 3.1%, p < 0.0001). The homozygote recessive variant of rs61745930 was present in 2.7% of the control group and 5.5% of the case group. Moreover, the minor allele frequencies of rs4646287 were 9.3 and 8.2% among the control and the case group, respectively (p = 0.767). Under the dominant (CC) genetic model of inheritance, rs2296651 was found to be protective of HBV infection [OR = 0.18 (0.07-0.44)], whereas under the co-dominant and additive model, rs2296651 was a potential risk factor for HBV infection [OR = 5.2 (95%CI: 2.1-12.8); 3.5 (95%CI: 1.6-7.6], respectively. Variants of rs61745930 and rs4646287 were not associated with HBV infection (p > 0.05). Polymorphisms in SLC10A1, however, did not show any significant association with HBV infectivity (p > 0.05).
The study highlights some polymorphism proof that variants rs2296651, rs61745930, and rs4646287 exist in HBV-infected individuals in Ghana. Although variant rs2296651 was found to be associated with HBV infection, this association warrants more studies. Polymorphisms in SLC10A1 were not associated with HBV infectivity among the Ghanaian population. Further investigation is warranted to assess the offensive role of the relationship between rs2296651 and HBV infectivity.
SLC10A1 基因编码 NTCP，乙型肝炎病毒 (HBV) 通过该受体进入肝细胞，这是病毒周期中必需的复制阶段。SLC10A1 的多态性变体在不同种族和国家的 HBV 感染、病毒清除、治疗结果和并发症中发挥作用。然而，在 HBV 流行的加纳人群中，尚未进行此类研究。因此，进行了一项探索性研究，以调查 SLC10A1 基因 (rs2296651、rs61745930 和 rs4646287) 中三个单核苷酸多态性 (SNP) 的存在，并评估了加纳人群中 HBV 感染的风险。
聚合酶链反应-限制性片段长度多态性 (PCR-RFLP) 方法用于确定 292 名参与者（包括 146 名 HBV 感染患者作为病例组和 146 名 HBV 未感染患者作为对照组）中 SNP 的存在。
与对照组相比，rs2296651 的次要等位基因频率 (T) 在病例组中存在显著较高的比例（11.6%比 3.1%，p<0.0001）。rs61745930 的纯合隐性变体在对照组中存在 2.7%，在病例组中存在 5.5%。此外，rs4646287 的次要等位基因频率分别为对照组和病例组的 9.3%和 8.2%（p=0.767）。在 rs2296651 的显性（CC）遗传模型下，发现 rs2296651 对 HBV 感染具有保护作用[OR=0.18（0.07-0.44）]，而在共显性和加性模型下，rs2296651 是 HBV 感染的潜在危险因素[OR=5.2（95%CI：2.1-12.8）；3.5（95%CI：1.6-7.6）]。rs61745930 和 rs4646287 的变体与 HBV 感染无关（p>0.05）。然而，SLC10A1 中的多态性与 HBV 感染性无显著关联（p>0.05）。
本研究强调了一些多态性证据，证明了加纳 HBV 感染个体中存在 rs2296651、rs61745930 和 rs4646287 变体。尽管发现变体 rs2296651 与 HBV 感染相关，但这种关联需要更多的研究。SLC10A1 中的多态性与加纳人群中的 HBV 感染性无关。需要进一步研究来评估 rs2296651 与 HBV 感染之间的关系的攻击性作用。
