Orsulak P J, Kenney J T, Debus J R, Crowley G, Wittman P D
University of Texas Southwestern Medical Center, Dallas.
Clin Chem. 1988 Sep;34(9):1875-8.
A procedure has been developed for measuring fluoxetine and its desmethyl metabolite, norfluoxetine, in serum by reversed-phase high-performance liquid chromatography (HPLC), with ultraviolet detection at 226 nm. Fluoxetine and norfluoxetine are isolated from serum by liquid-liquid extraction. They are then separated by HPLC and quantified, with reduced haloperidol as the internal standard. Fluoxetine, norfluoxetine, and the reduced haloperidol are separated from all interfering peaks in about 15 min. The standard curve is linear (r = 1.000) for both fluoxetine and norfluoxetine concentrations over the range of 25 to 800 micrograms/L. Between-run CVs for 60 and 200 micrograms/L controls (n = 8) were 6.8 and 4.1% for fluoxetine, and 8.8 and 6.2% for norfluoxetine, respectively. In a study of 24 patients with depression who were being treated with 20-60 mg of fluoxetine per day, fluoxetine and norfluoxetine concentrations in serum, measured during the last three weeks of treatment, were 47-469 micrograms/L and 52-446 micrograms/L, respectively.
已开发出一种通过反相高效液相色谱法（HPLC）在226nm处进行紫外检测来测定血清中氟西汀及其去甲基代谢物去甲氟西汀的方法。氟西汀和去甲氟西汀通过液液萃取从血清中分离出来。然后用HPLC分离并定量，以还原氟哌啶醇作为内标。氟西汀、去甲氟西汀和还原氟哌啶醇在约15分钟内与所有干扰峰分离。氟西汀和去甲氟西汀浓度在25至800微克/升范围内时，标准曲线呈线性（r = 1.000）。60和200微克/升对照品（n = 8）的批间变异系数，氟西汀分别为6.8%和4.1%，去甲氟西汀分别为8.8%和6.2%。在一项对24名每天接受20 - 60mg氟西汀治疗的抑郁症患者的研究中，治疗最后三周测得的血清中氟西汀和去甲氟西汀浓度分别为47 - 469微克/升和52 - 446微克/升。
