Holladay J W, Dewey M J, Yoo S D
College of Pharmacy, Nursing and Allied Health Sciences, Division of Pharmacy, Howard University, Washington, DC 20059, USA.
J Chromatogr B Biomed Sci Appl. 1997 Dec 19;704(1-2):259-63. doi: 10.1016/s0378-4347(97)00470-2.
A rapid and sensitive high-performance liquid chromatography assay method was developed to determine serum fluoxetine and norfluoxetine levels by single extraction of 0.1 ml of serum with sodium hydroxide. The mobile phase (55% acetonitrile-45% distilled water containing 10 mM aqueous triethylamine) was used to separate fluoxetine and norfluoxetine (25-1000 ng/ml, using clomipramine as the internal standard) by ultraviolet detection at 226 nm. The inter- and intra-day variabilities of fluoxetine and norfluoxetine were 13-18%, and the recoveries of both drugs exceeded 89%. This assay method was applied to a pharmacokinetic disposition study of fluoxetine in mice.
建立了一种快速灵敏的高效液相色谱测定方法，通过用氢氧化钠单次萃取0.1 ml血清来测定血清中氟西汀和去甲氟西汀的水平。流动相（含10 mM三乙胺水溶液的55%乙腈 - 45%蒸馏水）用于在226 nm处通过紫外检测分离氟西汀和去甲氟西汀（25 - 1000 ng/ml，以氯米帕明作为内标）。氟西汀和去甲氟西汀的日间和日内变异系数为13 - 18%，两种药物的回收率均超过89%。该测定方法应用于氟西汀在小鼠体内的药代动力学研究。
