Ezdini Khawla, Ben Salah-Abbès Jalila, Belgacem Héla, Mannai Marwa, Abbès Samir
Laboratory of Genetic, Biodiversity and Bio-resources Valorisation, University of Monastir, Monastir, Tunisia.
Laboratory of Genetic, Biodiversity and Bio-resources Valorisation, University of Monastir, Monastir, Tunisia; Higher Institute of Biotechnology of Béja, University of Jendouba, Jendouba, Tunisia.
Toxicon. 2020 Oct 15;185:46-56. doi: 10.1016/j.toxicon.2020.06.024. Epub 2020 Jul 1.
Fumonisin B1 (FB1) is a prevelant mycotoxin in our alimentary chain. It was produced by the fungi of the genus Fusarium verticillioides and Fusarium proliferatum. FB1 was playing as a competitive inhibitor of ceramide synthase; a key enzyme in the biosynthesis of sphingolipids. Indeed, it was associated with several affects in humans and livestock animals. The aim of our report was elucidated to evaluate the protective effects of Lactobacillus paracasei BEJ01 (LP) isolated from the traditional butter of Tunisia against the FB1 genotoxicity, hematoxicity, oxidative stress and histological damage in liver and kidney of BALB/c mice. Forty old week mice were randomly divided into four treatment groups (10 mice/group): Group 1: control; Group 2: LP (2 × 10 CFU/ml ~ 2 mg/kg p.c); Group 3: FB1 (100 μg/kg p.c.); Group 4: LP (2 × 10 CFU/ml ~ 2 mg/kg p.c) + FB1 (100 μg/kg p.c.). 48 h after the end of the treatment (10 days), the mice were sacrificed and the blood, liver and kidney were collected. The blood was used for hematological and biochemical studies. Liver and kidney samples were the target for evaluation of oxidative stress enzymes status and histopathological damage. The results indicated that FB1 was induced toxicities in the blood parameters and in liver and kidney tissues as well as in the profile of oxidative stress enzymes levels. The co-treatment with LP was found able to mitigate the FB1 toxicities by reduction of its bioavailability in the gastro intestinal tract. However, treatment with LP alone was safe and no sign of toxicity was showed. In Summary, the LP strain was able to prevent FB1 toxicities and indeed it could be exploited as one of the biological strategies for foodstuffs decontamination.
伏马菌素B1（FB1）是我们食物链中一种普遍存在的霉菌毒素。它由轮枝镰孢菌和层出镰孢菌属真菌产生。FB1作为神经酰胺合酶的竞争性抑制剂发挥作用；神经酰胺合酶是鞘脂生物合成中的关键酶。事实上，它与人类和家畜的多种影响有关。我们报告的目的是阐明从突尼斯传统黄油中分离出的副干酪乳杆菌BEJ01（LP）对BALB/c小鼠肝脏和肾脏中FB1的遗传毒性、血液毒性、氧化应激和组织学损伤的保护作用。40只老龄小鼠被随机分为四个治疗组（每组10只小鼠）：第1组：对照组；第2组：LP（2×10 CFU/ml2 mg/kg灌胃）；第3组：FB1（100μg/kg灌胃）；第4组：LP（2×10 CFU/ml2 mg/kg灌胃）+FB1（100μg/kg灌胃）。治疗结束（10天）后48小时，处死小鼠并采集血液、肝脏和肾脏。血液用于血液学和生化研究。肝脏和肾脏样本用于评估氧化应激酶状态和组织病理学损伤。结果表明，FB1在血液参数、肝脏和肾脏组织以及氧化应激酶水平方面均诱导了毒性。发现与LP联合治疗能够通过降低其在胃肠道中的生物利用度来减轻FB1的毒性。然而，单独用LP治疗是安全的，没有显示出毒性迹象。总之，LP菌株能够预防FB1的毒性，实际上它可以作为食品去污的生物策略之一加以利用。
