使用 JSPR 进行高分辨率单颗粒冷冻电镜重构。
High resolution single particle Cryo-EM refinement using JSPR.
机构信息
Department of Biological Sciences, Markey Center for Structural Biology, Purdue University, West Lafayette, IN, 47906, USA.
Department of Biological Sciences, Markey Center for Structural Biology, Purdue University, West Lafayette, IN, 47906, USA.
出版信息
Prog Biophys Mol Biol. 2021 Mar;160:37-42. doi: 10.1016/j.pbiomolbio.2020.05.006. Epub 2020 Jul 3.
Abstract
JSPR is a single particle cryo-EM image processing and 3D reconstruction software developed in the Jiang laboratory at Purdue University. It began as a few refinement scripts for symmetric and asymmetric reconstructions of icosahedral viruses, but has grown into a comprehensive suite of tools for building ab initio reconstructions, high resolution refinements of viruses, protein complexes of arbitrary symmetries including helical tubes/filaments, and image file handling utilities. In this review, we will present examples achieved using JSPR and demonstrate recently implemented features of JSPR such as multi-aberration "alignments" and automatic optimization of masking for the assessment of map resolution using "true" FSC.
摘要
JSPR 是一款单颗粒冷冻电镜图像处理和 3D 重建软件，由普渡大学江实验室开发。它最初是为二十面体病毒的对称和非对称重建开发的几个精修脚本，但现已发展成为一个用于从头开始构建重建、病毒高分辨率精修、任意对称的蛋白质复合物（包括螺旋管/丝）以及图像文件处理实用程序的综合工具集。在这篇综述中，我们将展示使用 JSPR 实现的示例，并演示 JSPR 的最新实现功能，例如多像差“对齐”和自动优化掩模，以使用“真实”FSC 评估映射分辨率。
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