Biocatalysis and Bioproducts Laboratory, Department of Biotechnology, Lorena School of Engineering, University of São Paulo, Lorena, SP, Brazil.
Biochemistry Laboratory, Department of Biotechnology, Lorena School of Engineering, University of São Paulo, Lorena, SP, Brazil.
J Biotechnol. 2020 Sep 10;321:35-47. doi: 10.1016/j.jbiotec.2020.07.001. Epub 2020 Jul 2.
This work investigated the integration of a two-stage hydrothermal treatment for production of xylooligosaccharides (XOS), a high-value product, into the isolation process of cellulose nanofibrils (CNF) from sugarcane bagasse. Under optimized conditions, the first stage yielded a XOS-rich, high purity hydrolysate and in the second stage only a xylose-rich hydrolysate could be obtained at high purity. The resulting solid cellulosic fraction was delignified and bleached to obtain a cellulose-rich pulp, which was mechanically defibrillated by disc ultra-refining to CNF. Except for the viscosity, the sugarcane CNF showed properties (i.e., thermal stability, crystallinity and diameter size) comparable or superior to the CNF prepared from commercial bleached eucalyptus Kraft pulp. In conclusion, the integration of the two-stage hydrothermal treatment is an efficient and promising strategy to obtain hemicellulose-derived high-value co-products in the process of isolating CNF. In addition, lignin was also recovered as a co-product with yield comparable to other biomass fractionation approaches.
本工作研究了两段式水热处理工艺在甘蔗渣纤维素纳米纤维（CNF）分离过程中生产高附加值木寡糖（XOS）的集成。在优化条件下，第一阶段得到了富含 XOS、高纯度的水解产物，而在第二阶段仅能以高纯度得到富含木糖的水解产物。得到的固体纤维素部分脱木质素和漂白得到富含纤维素的纸浆，然后通过盘式超微细化机械解纤得到 CNF。除了粘度外，甘蔗 CNF 的性能（即热稳定性、结晶度和直径尺寸）与商业漂白桉木硫酸盐浆制备的 CNF 相当或更优。总之，两段式水热处理的集成是一种从分离 CNF 过程中获得半纤维素衍生高附加值联产产物的有效且有前景的策略。此外，木质素也作为一种副产物回收，其产率与其他生物质分级方法相当。
