Lumsangkul Chompunut, Tapingkae Wanaporn, Sringarm Korawan, Jaturasitha Sanchai, Le Xuan Chinh, Wannavijit Supreya, Outama Piyatida, Van Doan Hien
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand.
Science and Technology Research Institute, Chiang Mai University, 239 Huay Keaw Rd., Suthep, Muang, Chiang Mai 50200, Thailand.
Animals (Basel). 2021 Jul 8;11(7):2035. doi: 10.3390/ani11072035.
We investigated, herein, the effects of dietary inclusion of sugarcane bagasse powder (SB) on Nile tilapia development, mucosal and serum immunities, and relative immune and antioxidant genes. Fish (15.12 ± 0.04 g) were provided a basal diet (SB0) or basal diet incorporated with SB at 10 (SB10), 20 (SB20), 40 (SB40), or 80 (SB80) g kg for 8 weeks. Our results demonstrated that the dietary incorporation of sugarcane bagasse powder (SB) at 20 and 40 g kg significantly ameliorated FW, WG, and SGR as opposed to fish fed basal, SB10, and SB80 diets. However, no significant changes in FCR and survivability were observed between the SB supplemented diets and the control (basal diet). The mucosal immunity exhibited significantly higher SMLA and SMPA activities ( < 0.005) in fish treated with SB diets after eight weeks. The highest SMLA and SMPA levels were recorded in fish fed SB80 followed by SB20, SB40, and SB10, respectively. For serum immunity, fish fed SB incorporated diets significantly ameliorated SL and RB levels ( < 0.05) compared with the control. However, SP was not affected by the inclusion of SB in any diet throughout the experiment. The expression of IL1, IL8, LBP, GSTa, GPX, and GSR genes in the fish liver was significantly increased in fish fed the SB20 and SB10 diets relative to the basal diet fed fish ( < 0.05); whereas only the IL8, LBP, and GPX genes in the intestines were substantially augmented via the SB20 and SB80 diets ( < 0.05). IL1 and GSR were not influenced by the SB incorporated diets ( > 0.05). In summary, sugarcane bagasse powder (SB) may be applied as a feed additive to improve growth performance, immune response, and immune and antioxidant-related gene expression in Nile tilapia.
在此，我们研究了日粮中添加甘蔗渣粉（SB）对尼罗罗非鱼生长发育、黏膜和血清免疫以及相关免疫和抗氧化基因的影响。为体重15.12±0.04克的鱼提供基础日粮（SB0）或添加了10（SB10）、20（SB20）、40（SB40）或80（SB80）克/千克SB的基础日粮，为期8周。我们的结果表明，与喂食基础日粮、SB10和SB80日粮的鱼相比，日粮中添加20和40克/千克甘蔗渣粉（SB）显著改善了鱼的终末体重（FW）、增重率（WG）和特定生长率（SGR）。然而，在添加SB的日粮组和对照组（基础日粮）之间，饲料系数（FCR）和存活率没有显著变化。八周后，用SB日粮处理的鱼的黏膜免疫表现出显著更高的溶菌酶活性（SMLA）和髓过氧化物酶活性（SMPA）（P<0.005）。最高的SMLA和SMPA水平分别出现在喂食SB80的鱼中，其次是SB20、SB40和SB10组。对于血清免疫，与对照组相比，喂食添加SB日粮的鱼显著改善了血清溶菌酶（SL）和补体C3（RB）水平（P<0.05）。然而，在整个实验过程中，任何日粮中添加SB均未影响血清总蛋白（SP）。与喂食基础日粮的鱼相比，喂食SB20和SB10日粮的鱼肝脏中白细胞介素1（IL1）、白细胞介素8（IL8）、脂多糖结合蛋白（LBP）、谷胱甘肽S-转移酶a（GSTa）、谷胱甘肽过氧化物酶（GPX）和谷胱甘肽还原酶（GSR）基因的表达显著增加（P<0.05）；而通过SB20和SB80日粮，肠道中只有IL8、LBP和GPX基因显著增加（P<0.05）。IL1和GSR不受添加SB日粮的影响（P>0.05）。总之，甘蔗渣粉（SB）可作为饲料添加剂，以改善尼罗罗非鱼的生长性能、免疫反应以及免疫和抗氧化相关基因的表达。
