α-1-抗糜蛋白酶对从人胃腺癌细胞中分离出的DNA引发酶活性的影响。
Effect of alpha-1-antichymotrypsin on activity of DNA primase isolated from human stomach adenocarcinoma cells.
作者信息
Takada S, Tsuda M, Yamamura M, Katsunuma T
机构信息
Department of Biochemistry, School of Medicine, Tokai University, Kanagawa, Japan.
出版信息
Biochem Int. 1988 May;16(5):949-54.
This paper describes an investigation into the effect of alpha-1-antichymotrypsin (ACT) on DNA primase. DNA primase was partially purified from human stomach carcinoma cells. It was found that poly(dC)-dependent DNa primase activity was inhibited by ACT and the inhibition was proportional to the concentration of the inhibitor. The inhibitory effect of ACT remained even after ACT lost most of its chymotrypsin-inhibitory activity by heat treatment. Poly(dT)-dependent primase activity was enhanced by the presence of ACT. The enhancement was effective up to a concentration of 1mg/ml.
本文描述了一项关于α-1-抗糜蛋白酶（ACT）对DNA引发酶作用的研究。DNA引发酶是从人胃癌细胞中部分纯化得到的。研究发现，依赖聚（dC）的DNA引发酶活性受到ACT的抑制，且这种抑制作用与抑制剂的浓度成正比。即使ACT经热处理失去大部分糜蛋白酶抑制活性后，其抑制作用依然存在。依赖聚（dT）的引发酶活性在ACT存在时增强。这种增强作用在浓度达到1mg/ml时仍有效。
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