Takada S, Tsuda M, Fujinami S, Yamamura M, Mitomi T, Katsunuma T
Cancer Res. 1986 Jul;46(7):3688-91.
Human stomach adenocarcinomas containing alpha-1-antichymotrypsin (ACT) in their cell nuclei were transplanted into nude mice. The presence of ACT was monitored using an immunohistochemical technique with horseradish peroxidase-labeled rabbit anti-ACT Fab' as well as single radial immunodiffusion. Two weeks after transplantation, ACT could be found neither in transplanted carcinoma cells nor in the sera of carcinoma-bearing nude mice. However, if human ACT was injected i.v., it could be detected in the transplanted carcinoma cell nuclei 2 h after injection. The ACT was detected immunohistochemically and was confirmed by biochemical fractionation using 125I-labeled ACT. On the other hand, the amount of ACT production was not sufficient to indicate biosynthesis. These results demonstrated that ACT detected in stomach carcinoma cell nuclei was not synthesized in carcinoma cells but was incorporated from the blood circulation.
将细胞核中含有α1抗糜蛋白酶（ACT）的人胃腺癌移植到裸鼠体内。使用辣根过氧化物酶标记的兔抗ACT Fab'免疫组织化学技术以及单向放射免疫扩散法监测ACT的存在。移植后两周，在移植的癌细胞和荷癌裸鼠的血清中均未发现ACT。然而，如果静脉注射人ACT，则在注射后2小时可在移植的癌细胞核中检测到。通过免疫组织化学检测到ACT，并通过使用125I标记的ACT进行生化分级分离得到证实。另一方面，ACT的产生量不足以表明其生物合成。这些结果表明，在胃癌细胞核中检测到的ACT不是在癌细胞中合成的，而是从血液循环中摄取的。
