Laboratory for Stem Cell & Regenerative Medicine, Natural and Medical Sciences Research Center, University of Nizwa, P. O. Box: 33, Nizwa, PC, 616, Oman.
Department of Mechanical and Industrial Engineering, Sultan Qaboos University, 123, Muscat, Oman.
Mol Biol Rep. 2020 Jul;47(7):5421-5430. doi: 10.1007/s11033-020-05632-y. Epub 2020 Jul 4.
Among medicinal plants, Acridocarpus orientalis (AO) possesses a remarkable anti-cancer potential, possibly because of its anti-oxidant property. In this study, the leaf and stem extracts from AO were assessed to find the bioactive compound with selective anti-cancer properties. The MTT viability and live and dead assays revealed that around 80% and 98% of 4T1 cells survival were declined after 48 h incubation with leaf and stem extracts, respectively. The leaf extract increased stem cell proliferation by 20% whereas the stem extract inhibited around 22% of stem cells proliferation after 48 h treatment. The live and dead assay of MSCs confirmed that 40% of the MSCs died when treated with AO stem extract. On the other hand, there were no dead cells after two days of treatment with the leaf extract. Followed by the induction of cell cycle arrest in G0/G1-phase, the real-time PCR demonstrated apoptosis properties in 4T1 cells through overexpression of Bax and down-regulation of BCL2 genes. Interestingly, within the pure compounds isolated from AO leaf extract, Morin was responsible for the inhibition of 4T1 cells proliferation as well as MSCs expansion, predicting to play an essential role in the treatment of cancer. The promising in vitro anti-cancer and stem cell-inductive properties of morin isolated from AO extract may provide a great potential to produce selective herbal derived drugs.
在药用植物中，东方飞龙果（AO）具有显著的抗癌潜力，可能是因为其具有抗氧化特性。在这项研究中，评估了 AO 的叶和茎提取物，以寻找具有选择性抗癌特性的生物活性化合物。MTT 活力和死活检测表明，在与叶和茎提取物孵育 48 小时后，约 4T1 细胞的存活率分别下降了 80%和 98%。叶提取物使干细胞增殖增加了 20%，而茎提取物在 48 小时处理后抑制了约 22%的干细胞增殖。MSCs 的死活检测证实，用 AO 茎提取物处理时，有 40%的 MSCs 死亡。另一方面，用叶提取物处理两天后，没有死亡细胞。实时 PCR 证实，细胞周期阻滞在 G0/G1 期，通过 Bax 的过表达和 BCL2 基因的下调，在 4T1 细胞中表现出凋亡特性。有趣的是，在从 AO 叶提取物中分离的纯化合物中，桑色素负责抑制 4T1 细胞的增殖和 MSCs 的扩增，这表明它在癌症治疗中起着重要作用。从 AO 提取物中分离出的 morin 具有体外抗肿瘤和诱导干细胞的特性，这为开发选择性的草药衍生药物提供了巨大的潜力。
