Department of Orthopaedics, People's Hospital of Ningxia Hui Autonomous Region (The First Affiliated Hospital of Northwest University For Nationalities), Yinchuan, Ningxia, China.
Department of orthopaedic trauma, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China.
Int J Immunopathol Pharmacol. 2020 Jan-Dec;34:2058738420962909. doi: 10.1177/2058738420962909.
Restoring bone defects are the major challenge facing clinical trial therapy, particularly skull related problems. Morin, a naturally occurring compound, has pro-osteogenesis. This research focuses on assessing the role of morin for its pro-osteogenesis activities. We utilized in vivo and in vitro models to investigate the molecular-level mechanisms of morin's osteoblastic biological activity. The effectiveness of morin on pro-osteogenesis (100 mg/kg/day) was assessed by monitoring modifications in the bone histomorphometry score, the development of immature osteoblasts from mesenchymal stems cells and improvements in the expression of pro-osteogenic cytokines in skull defected (SD) mice. Quantitative-PCR, Western blot analysis, and immunofluorescence were studied to investigate the signaling pathways. Morin has a substantial in vivo pro-osteogenesis effect which can facilitate the development of osteoblasts, the production of osteoblast related marker genes, and in vitro protein markers for osteoblasts. From a molecular biology standpoint, morin contributes to the development of osteoblasts and stimulation of the Wnt pathway with the activation and translocation of β-catenin nuclei. Our findings from the study revealed that morin may be a beneficial substitute for helping regenerate bone defects.
修复骨缺损是临床治疗面临的主要挑战，特别是与颅骨相关的问题。桑色素是一种天然存在的化合物，具有促进成骨作用。本研究专注于评估桑色素在促进成骨作用方面的作用。我们利用体内和体外模型来研究桑色素成骨细胞生物活性的分子水平机制。通过监测骨组织形态计量评分的变化、间充质干细胞中成骨细胞的不成熟发育以及颅骨缺损（SD）小鼠中成骨前细胞因子表达的改善，评估桑色素对成骨前作用（100mg/kg/天）。进行了定量 PCR、Western blot 分析和免疫荧光研究，以研究信号通路。桑色素具有显著的体内促骨生成作用，可促进成骨细胞的发育、成骨细胞相关标记基因的产生以及体外成骨细胞的蛋白质标记物。从分子生物学的角度来看，桑色素通过β-连环蛋白核的激活和易位，促进成骨细胞的发育和 Wnt 通路的刺激。我们的研究结果表明，桑色素可能是一种有助于骨缺损再生的有益替代物。
