Kosano H, Takatani O
Third Department of Internal Medicine, National Defense Medical College, Japan.
Cancer Res. 1988 Nov 1;48(21):6033-6.
The effects of 1-O-octadecyl-2-O-methyl-sn-glycero-3-phosphocholine (ET-18-OCH3), an alkyl lysophospholipid derivative, on the binding of epidermal growth factor (EGF) to human breast cancer cell lines (MCF-7, ZR-75-1, and BT-20), the human epidermoid cancer cell line (A431), and the rat fibroblast cell line (NIH3T3) were investigated. The addition of 10 micrograms/ml ET-18-OCH3 to the growth medium reduced the binding of EGF to hormone-dependent breast cancer cell lines (MCF-7 and ZR-75-1) and A431 but did not change that to the hormone-independent breast cancer cell line (BT-20). ET-18-OCH3 suppressed the EGF-binding prior to the onset of its inhibitory action on cell growth in MCF-7 and ZR-75-1. Scatchard plot analysis demonstrated that ET-18-OCH3 reduced the number of EGF receptor sites without affecting the affinity of EGF receptors in MCF-7 and ZR-75-1. Both EGF-binding and cell growth in NIH3T3 were not changed by treatment with 10 micrograms/ml ET-18-OCH3. These results suggest that ET-18-OCH3 inhibits the growth of hormone-dependent breast cancer cell lines (MCF-7 and ZR-75-1) by reducing the binding capacity of EGF receptors and consequently by disturbing the transfer of a variety of growth-promoting signals.
研究了烷基溶血磷脂衍生物1-O-十八烷基-2-O-甲基-sn-甘油-3-磷酸胆碱（ET-18-OCH3）对表皮生长因子（EGF）与人乳腺癌细胞系（MCF-7、ZR-75-1和BT-20）、人表皮样癌细胞系（A431）以及大鼠成纤维细胞系（NIH3T3）结合的影响。向生长培养基中添加10微克/毫升的ET-18-OCH3可降低EGF与激素依赖性乳腺癌细胞系（MCF-7和ZR-75-1）及A431的结合，但对激素非依赖性乳腺癌细胞系（BT-20）的结合无影响。在MCF-7和ZR-75-1中，ET-18-OCH3在开始抑制细胞生长之前就抑制了EGF结合。Scatchard图分析表明，ET-18-OCH3减少了MCF-7和ZR-75-1中EGF受体位点的数量，但不影响EGF受体的亲和力。用10微克/毫升的ET-18-OCH3处理对NIH3T3中的EGF结合和细胞生长均无影响。这些结果表明，ET-18-OCH3通过降低EGF受体的结合能力，进而干扰多种生长促进信号的传递，来抑制激素依赖性乳腺癌细胞系（MCF-7和ZR-75-1）的生长。
