Marolleau J P, Fondell J D, Malissen M, Trucy J, Barbier E, Marcu K B, Cazenave P A, Primi D
Unite d'Immunochimie Analytique, Departement d'Immunologie, CNRS 359, Paris, France.
Cell. 1988 Oct 21;55(2):291-300. doi: 10.1016/0092-8674(88)90052-9.
To determine whether T cell receptor genes follow the same principle of allelic exclusion as B lymphocytes, we have analyzed the rearrangements and expression of TCR alpha and beta genes in the progeny of the CD3+, CD4-/CD8- M14T line. Here, we show that this line can undergo secondary rearrangements that replace the pre-existing V alpha-J alpha rearrangements by joining an upstream V alpha gene to a downstream J alpha segment. Both the productively and nonproductively rearranged alleles in the M14T line can undergo secondary rearrangements while its TCR beta genes are stable. These secondary recombinations are usually productive, and new forms of TCR alpha polypeptides are expressed in these cells in association with the original C beta chain. Developmental control of this V alpha-J alpha replacement phenomenon could play a pivotal role in the thymic selection of the T cell repertoire.
为了确定T细胞受体基因是否遵循与B淋巴细胞相同的等位基因排斥原则，我们分析了CD3 +、CD4 - /CD8 - M14T系子代中TCRα和β基因的重排及表达情况。在此，我们表明该细胞系可发生二次重排，即通过将上游Vα基因与下游Jα片段连接，取代先前存在的Vα-Jα重排。M14T系中已发生有效重排和无效重排的等位基因均可发生二次重排，而其TCRβ基因则保持稳定。这些二次重组通常是有效的，并且在这些细胞中会表达新形式的TCRα多肽，并与原始的Cβ链结合。这种Vα-Jα替换现象的发育调控可能在T细胞库的胸腺选择中起关键作用。
