Cordiali-Fei P, Mottolese M, Tecce R, Natali P, Ferrone S
Laboratory of Immunology, S. Gallicano Institute of Dermatology, Rome, Italy.
Cell Immunol. 1988 Oct 1;116(1):149-62. doi: 10.1016/0008-8749(88)90217-1.
Six out of eight human melanoma cell lines were found to be able to function as accessory cells in PHA-induced proliferation of autologous and allogeneic T cells. The accessory cell function of the melanoma cell lines appears to be similar to that of monocytes, requires the presence of viable cells, and does not correlate with the cell surface binding sites for PHA and with the level of expression of HMW-MAA and of HLA Class I antigens. HLA Class II antigens do not appear to play a major role in these phenomena, since there is no relationship between level of expression of HLA Class II antigens and accessory cell function of melanoma cells. Furthermore, addition of anti-HLA Class II monoclonal antibodies does not affect proliferation of T cells stimulated with PHA in the presence of melanoma cells with accessory cell function. Although melanoma cells exert accessory cell function, functional and immunological assays did not detect IL-1 in the spent medium of the melanoma cell lines. Furthermore, Northern blotting analysis with IL-1 alpha and IL-1 beta probes did not detect IL-1-specific mRNA in melanoma cell lines. These results suggest that PHA-induced proliferation of T cells in the presence of melanoma cells can bypass the requirement for IL-1 or utilizes factors other than IL-1.
在8种人类黑色素瘤细胞系中，发现有6种能够在PHA诱导的自体和异体T细胞增殖过程中发挥辅助细胞的功能。黑色素瘤细胞系的辅助细胞功能似乎与单核细胞的功能相似，需要活细胞的存在，并且与PHA的细胞表面结合位点以及高分子量黑色素瘤相关抗原（HMW-MAA）和HLA I类抗原的表达水平无关。HLA II类抗原似乎在这些现象中不起主要作用，因为HLA II类抗原的表达水平与黑色素瘤细胞的辅助细胞功能之间没有关系。此外，添加抗HLA II类单克隆抗体不会影响在具有辅助细胞功能的黑色素瘤细胞存在的情况下PHA刺激的T细胞增殖。尽管黑色素瘤细胞发挥辅助细胞功能，但功能和免疫学检测在黑色素瘤细胞系的培养上清中未检测到IL-1。此外，用IL-1α和IL-1β探针进行的Northern印迹分析在黑色素瘤细胞系中未检测到IL-1特异性mRNA。这些结果表明，在黑色素瘤细胞存在的情况下PHA诱导的T细胞增殖可以绕过对IL-1的需求或利用IL-1以外的因子。
