Lyt2-、L3T4-胸腺细胞的体外增殖显示出对白介素1的反应性。
Proliferation in vitro of Lyt2-,L3T4- thymocytes shows responsiveness to interleukin 1.
作者信息
Chaudhri G, Clark I A, Ceredig R
机构信息
Department of Experimental Pathology, John Curtin School of Medical Research, Canberra, Australia.
出版信息
Clin Exp Immunol. 1988 Jul;73(1):51-6.
The responsiveness of adult mouse thymocytes to interleukin 1 (IL-1) was investigated. When stimulated with suboptimal concentrations of ionomycin and phorbol myristate acetate (PMA), the immature subpopulation of Lyt2-,L3T4- (2-4-) thymocytes responded to exogenous, purified IL-1 in a dose-dependent manner. In contrast, mature lymph node (LN) T cells were unresponsive to exogenous IL-1 under similar conditions. The proliferation of 2-4- thymocytes, which comprise 2-3% of cells in the adult mouse thymus, was independent of exogenous IL-2 and accounted for essentially all the 3H-thymidine incorporation by unfractionated thymocytes in response to IL-1. Optimally stimulated 2-4- cells did not show responsiveness to exogenous IL-1, and 48 h supernatants from these cultures were found to contain IL-1 like activity. Taken together, the data point to a role for IL-1 in the proliferation of immature 2-4- cells in the thymus.
对成年小鼠胸腺细胞对白介素1（IL-1）的反应性进行了研究。当用次优浓度的离子霉素和佛波酯肉豆蔻酸酯（PMA）刺激时，Lyt2-、L3T4-（2-4-）胸腺细胞的未成熟亚群对外源性纯化的IL-1呈剂量依赖性反应。相比之下，在类似条件下，成熟的淋巴结（LN）T细胞对外源性IL-1无反应。占成年小鼠胸腺细胞2%-3%的2-4-胸腺细胞的增殖不依赖外源性IL-2，并且基本上占未分级胸腺细胞对IL-1反应所产生的所有3H-胸腺嘧啶核苷掺入量。最佳刺激的2-4-细胞对外源性IL-1无反应，并且发现这些培养物48小时的上清液含有IL-1样活性。综上所述，数据表明IL-1在胸腺中未成熟2-4-细胞的增殖中起作用。
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