Ceredig R
J Immunol. 1986 Oct 1;137(7):2260-7.
When 14 day fetal mouse thymocytes, which are phenotypically Lyt-2-/L3T4-(2-4-), were stimulated in vitro with a combination of phorbol myristate acetate (PMA) and the calcium ionophore ionomycin, they proliferated without addition of exogenous interleukins and/or growth factors. Addition of exogenous IL 2 resulted in a slight enhancement of fetal thymocyte proliferation. By using factor-dependent indicator cell lines, this proliferation was shown to be accompanied by the production of IL 2 and IL 3. However, phenotypic analysis by using flow microfluorometry and monoclonal antibodies to Lyt-2 and L3T4 showed little differentiation among proliferating 2-4-fetal thymocytes. Interestingly, the in vitro growth of PMA + ionomycin-stimulated fetal thymocytes appeared to be IL 2 dependent in that it was inhibited by a monoclonal antibody to the IL 2 receptor. The results obtained with fetal thymocytes were compared with those obtained when using 2-4- thymocytes from adult mice.
当用佛波醇肉豆蔻酸酯乙酸酯（PMA）和钙离子载体离子霉素的组合在体外刺激表型为Lyt-2-/L3T4-（2-4-）的14天龄胎鼠胸腺细胞时，它们在不添加外源性白细胞介素和/或生长因子的情况下增殖。添加外源性IL-2导致胎鼠胸腺细胞增殖略有增强。通过使用依赖因子的指示细胞系，这种增殖显示伴随着IL-2和IL-3的产生。然而，通过流式微量荧光测定法以及针对Lyt-2和L3T4的单克隆抗体进行的表型分析显示，增殖的2-4-胎鼠胸腺细胞之间几乎没有分化。有趣的是，PMA +离子霉素刺激的胎鼠胸腺细胞的体外生长似乎依赖于IL-2，因为它被抗IL-2受体的单克隆抗体所抑制。将胎鼠胸腺细胞获得的结果与使用成年小鼠的2-4-胸腺细胞时获得的结果进行了比较。
