Suenaga R, Abdou N I
Department of Internal Medicine, University of Kansas Medical Center, Kansas City 66103.
Clin Immunol Immunopathol. 1988 Nov;49(2):251-60. doi: 10.1016/0090-1229(88)90115-8.
Sharing of private idiotypes (Id) on human polyclonal IgG anti-double-stranded DNA (dsDNA) with coexisting IgM anti-dsDNA was investigated using rabbit (R) anti-Id raised against IgG anti-dsDNA. The R-anti-Id showed specificity to private Id in or near the antigen-binding sites. The R-anti-Id poorly bound to the immobilized enriched IgM anti-dsDNA preparation but significantly bound to IgG anti-dsDNA preparation by a direct-binding ELISA (0.020 OD vs 0.295 OD, respectively). The R-anti-Id poorly inhibited the binding of IgM anti-dsDNA to immobilized dsDNA but significantly inhibited the binding of IgG anti-dsDNA to dsDNA (6% vs 55% inhibition, respectively). This was confirmed by poor inhibition of binding of the R-anti-Id to immobilized IgG anti-dsDNA by the enriched IgM anti-dsDNA preparation (maximum of 26% inhibition at 50 micrograms/ml). Nonsharing of private Id between IgG and coexisting IgM anti-dsDNA may represent the idiotypic diversity of human anti-DNA antibodies secondary to the frequent occurrence of somatic mutation on anti-DNA antibody during class switching.
利用针对人多克隆IgG抗双链DNA（dsDNA）产生的兔抗独特型（Id），研究了共存IgM抗dsDNA上的人源多克隆IgG抗dsDNA的私有独特型共享情况。兔抗独特型对抗原结合位点内或附近的私有独特型具有特异性。通过直接结合酶联免疫吸附测定法（ELISA），兔抗独特型与固定化的富集IgM抗dsDNA制剂结合不佳，但与IgG抗dsDNA制剂显著结合（分别为0.020 OD对0.295 OD）。兔抗独特型对IgM抗dsDNA与固定化dsDNA的结合抑制作用不佳，但对IgG抗dsDNA与dsDNA的结合有显著抑制作用（分别为6%对55%抑制）。富集的IgM抗dsDNA制剂对兔抗独特型与固定化IgG抗dsDNA的结合抑制作用不佳（50微克/毫升时最大抑制率为26%），这证实了上述情况。IgG与共存的IgM抗dsDNA之间不存在私有独特型共享，这可能代表了在类别转换过程中，由于抗DNA抗体上频繁发生体细胞突变，导致人抗DNA抗体的独特型多样性。
