Suenaga R, Abdou N I
Immunology Research Laboratory, St. Luke's Hospital, Kansas City, MO 64111.
J Autoimmun. 1992 Jun;5(3):379-92. doi: 10.1016/0896-8411(92)90150-o.
The possibility that idiotypes (Ids) defined on anti-double stranded DNA (dsDNA) antibodies during active and inactive stages of lupus (1/84 Id and 4/90 Id, respectively) were expressed on anti-DNA antibodies during a subsequent active period (9/90) of the disease was investigated in a lupus patient with lupus cerebritis. Using rabbit (R)-anti-Ids specific to 1/84 Id and 4/90 Id in inhibition assays, the 4/90 Id was shown to be expressed on the framework regions of anti-single stranded DNA (ssDNA) but poorly on co-existing anti-dsDNA antibodies of active (9/90) stage. The 1/84 Id was poorly expressed on both types of 9/90 anti-DNA antibodies. While the 9/90 anti-ssDNA significantly bound to immobilized ssDNA and several single-stranded polynucleotides, only ssDNA inhibited the binding of the anti-ssDNA to ssDNA, suggesting its monospecificity toward ssDNA. Western blot analysis following isoelectric focusing showed that a spectrotype pattern of 4/90 Id-positive 9/90 anti-ssDNA IgG was similar to that of the 4/90 anti-dsDNA, suggesting that they are of related clonal origin. The present study suggests the idiotypic heterogeneity of anti-DNA antibodies and the shift of antigen specificity within an idiotypically related anti-DNA population during exacerbation of the disease.
在一名患有狼疮性脑病的狼疮患者中，研究了在狼疮活动期和非活动期（分别为1/84 Id和4/90 Id）定义的抗双链DNA（dsDNA）抗体上的独特型（Ids）在疾病随后的活动期（9/90）是否在抗DNA抗体上表达。在抑制试验中使用对1/84 Id和4/90 Id特异的兔（R）-抗Ids，结果显示4/90 Id在抗单链DNA（ssDNA）的构架区表达，但在活动期（9/90）共存的抗dsDNA抗体上表达较弱。1/84 Id在9/90型的两种抗DNA抗体上表达均较弱。虽然9/90抗ssDNA与固定化的ssDNA和几种单链多核苷酸有显著结合，但只有ssDNA能抑制抗ssDNA与ssDNA的结合，提示其对ssDNA具有单特异性。等电聚焦后的蛋白质印迹分析显示，4/90 Id阳性的9/90抗ssDNA IgG的光谱型模式与4/90抗dsDNA相似，提示它们具有相关的克隆起源。本研究提示抗DNA抗体的独特型异质性以及在疾病加重期间独特型相关抗DNA群体内抗原特异性的转变。
