Stiller-Winkler R, Radaszkiewicz T, Gleichmann E
Institute of Hygiene, University of Düsseldorf, F.R.G.
Int J Immunopharmacol. 1988;10(4):475-84. doi: 10.1016/0192-0561(88)90136-1.
In the present study we screened mice from 22 different inbred strains for potential differences in their immunological reaction to HgCl2. As a rapid screening test, we used the popliteal lymph node assay (PLNA). Mice were injected s.c. into one hind footpad with 3-60 micrograms of Hg2+, given as HgCl2 in phosphate-buffered saline (PBS); contralateral hindfeet remained uninjected. Control mice received PBS alone. On day 6 the weights of the draining and contralateral PLN were determined and the PLN index calculated. While we found linear dose-response curves in some strains, these curves had a different shape in others. Out of the total of 21 euthymic strains tested only strain DBA/2 (H-2d) proved to be a nonresponder to HgCl2; it remained a nonresponder over the whole dose range (3-180 micrograms Hg2+) and period of time (days 2-12) studied. The other H-2d strains tested, i.e. NZB, BALB/c and B10.D2/n, showed absent or low PLN responses only in the lower dose range (3-30 micrograms Hg2+). F1 hybrids of strain DBA/2 and the responder strain C57BL/6 gave an intermediate response. While C3H nu/nu mice failed to respond to HgCl2, C3H +/nu mice did. The weight increase of the draining PLN after HgCl2 injection was preceded in time by an increased 3H thymidine uptake by the PLN. Histologically, enlarged PLNs revealed increased cellularity in both the T-cell and the B-cell areas. When CH3HgCl, instead of HgCl2, was injected all three strains tested, including DBA/2, responded by PLN enlargement. We conclude that (1) HgCl2 is an immunostimulatory agent in mice in that it induces T-cell-dependent enlargement of the draining PLN upon local injection, (2) there are striking genetic differences between inbred strains of mice in the PLN response to HgCl2, but these differences are not paralleled by similar differences in the response to CH3HgCl, (3) responsiveness to HgCl2 appears to be inherited in a codominant fashion, and (4) there is suggestive evidence that the observed genetic differences are determined by both H-2 and non-H-2 genes.
在本研究中，我们对来自22个不同近交系的小鼠进行了筛选，以寻找它们对氯化汞免疫反应的潜在差异。作为一种快速筛选试验，我们采用了腘窝淋巴结试验（PLNA）。将小鼠的一侧后足垫皮下注射3 - 60微克汞离子（以磷酸缓冲盐水（PBS）中的氯化汞形式给予）；对侧后足不注射。对照小鼠仅接受PBS。在第6天，测定引流和对侧腘窝淋巴结的重量，并计算腘窝淋巴结指数。虽然我们在一些品系中发现了线性剂量反应曲线，但在其他品系中这些曲线形状不同。在总共测试的21个正常胸腺品系中，只有DBA/2（H - 2d）品系被证明对氯化汞无反应；在整个研究的剂量范围（3 - 180微克汞离子）和时间段（第2 - 12天）内它一直无反应。所测试的其他H - 2d品系，即NZB、BALB/c和B10.D2/n，仅在较低剂量范围（3 - 30微克汞离子）显示出无或低的腘窝淋巴结反应。DBA/2品系与反应品系C57BL/6的F1杂种表现出中等反应。虽然C3H裸鼠对氯化汞无反应，但C3H +/裸鼠有反应。注射氯化汞后引流腘窝淋巴结重量增加之前，腘窝淋巴结的3H胸苷摄取量增加。组织学上，肿大的腘窝淋巴结在T细胞和B细胞区域均显示细胞增多。当注射甲基氯化汞而非氯化汞时，所测试的包括DBA/2在内的所有三个品系均通过腘窝淋巴结肿大作出反应。我们得出以下结论：（1）氯化汞在小鼠中是一种免疫刺激剂，因为局部注射后它会诱导引流腘窝淋巴结的T细胞依赖性肿大；（2）小鼠近交系在对氯化汞的腘窝淋巴结反应方面存在显著的遗传差异，但这些差异在对甲基氯化汞的反应中没有类似的差异；（3）对氯化汞的反应性似乎以共显性方式遗传；（4）有提示性证据表明观察到的遗传差异由H - 2和非H - 2基因共同决定。
