Olagaray Katie E, Bradford Barry J, Sordillo Lorraine M, Gandy Jeffery C, Mamedova Laman K, Swartz Turner H, Jackson Trey D, Persoon Emma K, Shugart Caitlin S, Youngs Curtis R
Department of Animal Sciences and Industry, Kansas State University, Manhattan, 66506 USA.
College of Veterinary Medicine, Michigan State University, 2265K Anthony Hall, East Lansing, MI 48824-1225 USA.
J Anim Sci Biotechnol. 2020 Jul 1;11:68. doi: 10.1186/s40104-020-00473-y. eCollection 2020.
Postpartum inflammation is a natural and necessary response; however, a dysfunctional inflammatory response can be detrimental to animal productivity. The objective of this study was to determine the effects of a non-steroidal anti-inflammatory drug (meloxicam) on ewe postpartum inflammatory response, ewe plasma polyunsaturated fatty acid and oxylipid concentrations, and lamb growth.
After lambing, 36 Hampshire and Hampshire × Suffolk ewes were sequentially assigned within type of birth to control ( = 17) or meloxicam orally administered on d 1 and 4 of lactation (MEL; 90 mg, = 19). Milk and blood samples were collected on d 1 (prior to treatment) and d 4. Milk glucose-6-phosphate was not affected by MEL. Plasma haptoglobin (Hp) concentrations were less for MEL ewes; control ewes with greater d 1 Hp concentrations had elevated Hp on d 4, but this was not the case for MEL-treated ewes. Treatment with MEL increased plasma arachidonic acid concentration by more than 4-fold in ewes rearing singles but decreased concentrations of 9,10-dihydroxyoctadecenoic acid, prostaglandin F, 8-iso-prostaglandin E, and 8,9-dihydroxyeicosatetraenoic acid. Nine oxylipids in plasma had interactions of treatment with d 1 Hp concentration, all of which revealed positive associations between d 1 Hp and d 4 oxylipid concentrations for CON, but neutral or negative relationships for MEL. MEL decreased 13-hydroxyoctadecadienoic acid:13-oxooctadecadienoic acid ratio and tended to increase 9-hydroxyoctadecadienoic acid:9-oxooctadecadienoic acid ratio (both dependent on d 1 values), indicating progressive metabolism of linoleic acid-derived oxylipids occurred by enzymatic oxidation after MEL treatment. Meloxicam reduced oxylipids generated across oxygenation pathways, potentially due to an improved redox state.
Postpartum MEL treatment of ewes decreased plasma concentrations of Hp and several oxylipids, with the greatest impact in ewes with biomarkers reflecting a greater inflammatory state before treatment. Anti-inflammatory strategies may help resolve excessive postpartum inflammation in some dams.
产后炎症是一种自然且必要的反应；然而，功能失调的炎症反应可能对动物生产力有害。本研究的目的是确定一种非甾体抗炎药（美洛昔康）对母羊产后炎症反应、母羊血浆多不饱和脂肪酸和氧化脂质浓度以及羔羊生长的影响。
产羔后，36只汉普夏和汉普夏×萨福克母羊按出生类型依次分为对照组（n = 17）或在泌乳第1天和第4天口服美洛昔康的组（MEL；90 mg，n = 19）。在第1天（治疗前）和第4天采集牛奶和血液样本。牛奶中的葡萄糖-6-磷酸不受美洛昔康影响。MEL组母羊的血浆触珠蛋白（Hp）浓度较低；第1天Hp浓度较高的对照组母羊在第4天Hp升高，但美洛昔康治疗的母羊并非如此。美洛昔康处理使单胎饲养母羊的血浆花生四烯酸浓度增加了4倍多，但降低了9,10-二羟基十八碳烯酸、前列腺素F、8-异前列腺素E和8,9-二羟基二十碳四烯酸的浓度。血浆中的9种氧化脂质存在处理与第1天Hp浓度的相互作用，所有这些相互作用都显示对照组第1天Hp与第4天氧化脂质浓度呈正相关，而美洛昔康组呈中性或负相关。美洛昔康降低了13-羟基十八碳二烯酸:13-氧代十八碳二烯酸的比值，并倾向于增加9-羟基十八碳二烯酸:9-氧代十八碳二烯酸的比值（两者均取决于第1天的值），表明美洛昔康处理后，亚油酸衍生的氧化脂质通过酶促氧化发生了渐进性代谢。美洛昔康减少了通过氧化途径产生的氧化脂质，这可能是由于氧化还原状态的改善。
产后对母羊进行美洛昔康处理可降低血浆中Hp和几种氧化脂质的浓度，对治疗前生物标志物反映炎症状态较高的母羊影响最大。抗炎策略可能有助于解决一些母羊产后的过度炎症反应。
