Chetty S Shashank, Praneetha S, Vadivel Murugan A, Govarthanan Kavitha, Verma Rama S
Advanced Functional Nanostructured Materials Laboratory, Centre for Nanoscience and Technology, Madanjeet School of Green Energy Technologies, Pondicherry University (A Central University), Puducherry, 605014, India.
Stem Cell and Molecular Biology Laboratory, Bhupat and Jyoti Mehta School of Biosciences, Department of Biotechnology, Indian Institute of Technology-Madras (IIT-M), Chennai, 600036, India.
Adv Biosyst. 2019 Jan;3(1):e1800127. doi: 10.1002/adbi.201800127. Epub 2018 Oct 29.
Near-infrared (NIR) fluorescent CuInS -ZnS nanocrystals (CIZS NCs) are synthesized via an ultra-fast, non-injection microwave (MW)-assisted nanoalloying process at 230 ºC within 5 min using 1-dodecanethiol (DDT) as both the sulfur source and solvent under solvothermal (ST) condition. The structural and surface analyses reveal that DDT-functionalized CIZS NCs exhibit quasi-pyramids of tetragonal-phase with well-defined facets. The DDT-functionalized CIZS NCs present a photoluminescence quantum yield (PLQY) of 76% and a long-lived fluorescence lifetime of ≈0.6 µs in organic-phase. Subsequently, DDT-functionalized CIZS NCs are phase-transferred via ligand-exchange using 11-mercaptoundecanoic acid (MUA) into water-soluble MUA-CIZS NCs that exhibit a substantial PLQY of 55%. In addition, the NIR-fluorescent MUA-functionalized CIZS NCs in conjugation with folic acid (FA), as a tumor-targeting ligand, demonstrates enhanced tumor-targeted imaging ability. The FA-MUA-CIZS NC conjugates exhibit a cell viability of ≈75% even at the highest concentration of 1 mg mL and a labeling efficiency of 95.4%. The in vivo imaging results corroborate that FA-MUA-CIZS NCs conjugates are actively targeted to folate receptor-positive B16F10 tumor-bearing C57BL/6 mice in 2 h. The histopathological and hematological studies confirm no significant changes in tissue architecture and blood biochemical parameters. The confocal microscopy studies reveal deep penetration and uniform distribution of FA-MUA-CIZS NCs conjugates in subcutaneous melanoma.
通过超快、非注入式微波（MW）辅助的纳米合金化过程，在230℃下于5分钟内在溶剂热（ST）条件下，使用1-十二烷硫醇（DDT）作为硫源和溶剂合成近红外（NIR）荧光铜铟硫-硫化锌纳米晶体（CIZS NCs）。结构和表面分析表明，DDT功能化的CIZS NCs呈现出具有清晰晶面的四方相准金字塔形状。DDT功能化的CIZS NCs在有机相中具有76%的光致发光量子产率（PLQY）和约0.6微秒的长荧光寿命。随后，通过使用11-巯基十一烷酸（MUA）进行配体交换，将DDT功能化的CIZS NCs相转移到水溶性的MUA-CIZS NCs中，其PLQY为55%。此外，与作为肿瘤靶向配体的叶酸（FA）共轭的近红外荧光MUA功能化CIZS NCs表现出增强的肿瘤靶向成像能力。即使在最高浓度1毫克/毫升时，FA-MUA-CIZS NC共轭物的细胞活力约为75%，标记效率为95.4%。体内成像结果证实，FA-MUA-CIZS NC共轭物在2小时内可主动靶向叶酸受体阳性的荷B16F10肿瘤的C57BL/6小鼠。组织病理学和血液学研究证实组织结构和血液生化参数无明显变化。共聚焦显微镜研究揭示了FA-MUA-CIZS NC共轭物在皮下黑色素瘤中的深度渗透和均匀分布。
