三磷酸腺苷（ATP）和其他腺嘌呤化合物可增强大鼠心脏的机械活动并增加三磷酸肌醇的生成。

ATP and other adenine compounds increase mechanical activity and inositol trisphosphate production in rat heart.

Legssyer A, Poggioli J, Renard D, Vassort G

Unité de Recherches de Physiologie Cellulaire Cardiaque INSERM U-241, Université Paris, Orsay, France.

J Physiol. 1988 Jul;401:185-99. doi: 10.1113/jphysiol.1988.sp017157.

Abstract

The effects of adenosine 5'-triphosphate (ATP) and other adenine compounds were examined on rat papillary and right ventricular muscles in the presence of 10 microM-propranolol, 10 microM-atropine and 0.1 microM-prazosin or 10 microM-phentolamine. 2. Adenosine, adenosine 5'-monophosphate (AMP), adenosine 5'-diphosphate (ADP), ATP and alpha,beta-methylene ATP (APCPP) produced small positive inotropic effects, sometimes preceded by transient negative effects. 3. 8-Phenyltheophylline (8-PT), a P1-purinoceptor antagonist antagonized the negative effects and increased the positive inotropy induced by ATP and adenosine. 4. In the presence of APCPP, a P2-purinergic agonist, ATP had only negative inotropic effects. 5. Adenosine and ATP increased inositol 1, 4, 5- and inositol 1, 3, 4-trisphosphate as well as inositol mono- and bisphosphate formation. Maximal effects were obtained at concentrations of 0.5 mM. 6. APCPP increased inositol phosphate formation while 8-PT did not prevent the effects of adenosine and ATP. 7. It is suggested that P2-purinoceptor activation induces both a positive inotropy and an increase in inositol-lipid metabolism in rat ventricular muscles.

摘要

在存在10微摩尔普萘洛尔、10微摩尔阿托品和0.1微摩尔哌唑嗪或10微摩尔酚妥拉明的情况下，研究了5'-三磷酸腺苷（ATP）和其他腺嘌呤化合物对大鼠乳头肌和右心室肌的作用。2. 腺苷、5'-单磷酸腺苷（AMP）、5'-二磷酸腺苷（ADP）、ATP和α,β-亚甲基ATP（APCPP）产生小的正性肌力作用，有时之前会有短暂的负性作用。3. P1嘌呤受体拮抗剂8-苯基茶碱（8-PT）拮抗负性作用，并增强ATP和腺苷诱导的正性肌力作用。4. 在P2嘌呤能激动剂APCPP存在的情况下，ATP仅产生负性肌力作用。5. 腺苷和ATP增加了肌醇1,4,5-三磷酸和肌醇1,3,4-三磷酸以及肌醇单磷酸和双磷酸的形成。在0.5毫摩尔浓度时获得最大效应。6. APCPP增加了肌醇磷酸的形成，而8-PT并未阻止腺苷和ATP的作用。7. 提示P2嘌呤受体激活在大鼠心室肌中既诱导正性肌力作用，又诱导肌醇脂质代谢增加。