Department of Anesthesiology and Pain Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea.
Department of Anesthesiology and Pain Medicine, Soonchunhyang University Cheonan Hospital, Soonchunhyang University College of Medicine, Cheonan, Korea.
J Korean Med Sci. 2020 Jul 6;35(26):e225. doi: 10.3346/jkms.2020.35.e225.
Spinal nerve ligation (SNL) model is one of the representative models of the neuropathic pain model. Neuropathic pain in a chronic post-ischemic pain (CPIP) mimics the symptoms of complex regional pain syndrome (CRPS). The administration of polydeoxyribonucleotide (PDRN), which has regenerative and anti-inflammatory effects, has been studied and is used in clinical practice treating various diseases. However, the analgesic effect of PDRN in a neuropathic pain or CRPS model remains unknown.
PDRN (3.3, 10, and 20 mg/kg) was administered into the subcutaneous (SC) layer of the hind paws of SNL and CPIP models. Mechanical anti-allodynic effects were then investigated using the von Frey test. In the immunohistochemical examination, dorsal root ganglia (DRG) and the spinal cord were harvested and examined for the expression of glial fibrillary acidic protein (GFAP) after the 20 mg PDRN injection.
Mechanical allodynia was significantly alleviated by administration of PDRN in SNL and CPIP mice at all of the time point. As the dose of PDRN increased, the effect was greater. The 20 mg PDRN injection was found to have the most effective anti-allodynic effect. The increased expression of GFAP in DRG and the spinal cord of SNL and CPIP model decreased following the administration of PDRN than vehicle.
SC administration of PDRN results in the attenuation of allodynia and activation of astrocytes in neuropathic pain or CRPS models. We propose that PDRN can have significant potential advantages in neuropathic pain treatment.
脊神经结扎（SNL）模型是神经病理性疼痛模型的代表性模型之一。慢性缺血后疼痛（CPIP）中的神经病理性疼痛模拟了复杂性区域疼痛综合征（CRPS）的症状。具有再生和抗炎作用的聚脱氧核糖核苷酸（PDRN）已被研究并用于治疗各种疾病的临床实践。然而，PDRN 在神经病理性疼痛或 CRPS 模型中的镇痛作用尚不清楚。
将 PDRN（3.3、10 和 20mg/kg）注入 SNL 和 CPIP 模型的足底皮下（SC）层。然后使用 von Frey 测试研究机械性抗痛觉过敏作用。在免疫组织化学检查中，在注射 20mg PDRN 后收获背根神经节（DRG）和脊髓，并检查胶质纤维酸性蛋白（GFAP）的表达。
在 SNL 和 CPIP 小鼠中，PDRN 的给药显著缓解了机械性痛觉过敏。随着 PDRN 剂量的增加，效果更大。发现 20mg PDRN 注射具有最有效的抗痛觉过敏作用。DRG 和 SNL 及 CPIP 模型脊髓中 GFAP 的表达增加，在给予 PDRN 后比给予载体时减少。
SC 给予 PDRN 可减轻神经病理性疼痛或 CRPS 模型中的痛觉过敏和星形胶质细胞的激活。我们提出 PDRN 在治疗神经病理性疼痛方面具有显著的潜在优势。
