Diop Khoudia, Cadoret Frederic, Nguyen Thi Tien, Baudoin Jean-Pierre, Armstrong Nicholas, Raoult Didier, Bretelle Florence, Fournier Pierre-Edouard, Fenollar Florence
Aix-Marseille Univ, IRD, AP-HM, SSA, VITROME, Marseille, France.
IHU-Méditerranée Infection, Marseille, France.
Int J Syst Evol Microbiol. 2020 Jul;70(7):4091-4097. doi: 10.1099/ijsem.0.004106.
A Gram-stain-positive anaerobic rod-shaped bacterium, designated strain Marseille-P3275, was isolated using culturomics from the vaginal discharge of healthy French woman. Marseille-P3275 was non-motile and did not form spores. Cells had neither catalase nor oxidase activity. The major fatty acids were C (29 %), Cω9 (18 %), and iso-C (17 %). The genomic DNA G+C content was 50.64 mol%. The phylogenetic analysis based on 16S rRNA gene sequence indicated that Marseille-P3275 was related to members of the family (between 90.32-92.92 % sequence similarity) with formation of a clade with the monospecific genus (type species ). On the basis of these phylogenetic and phenotypic differences, Marseille-P3275 was classified in a novel genus, , as gen. nov., sp. nov. The type strain is Marseille-P3275 (=CSUR P3275=CECT 9677).
从一名健康法国女性的阴道分泌物中，采用 culturomics 方法分离出一株革兰氏阳性厌氧杆状细菌，命名为马赛 - P3275 菌株。马赛 - P3275 菌株无运动性，不形成芽孢。细胞既无过氧化氢酶活性也无氧化酶活性。主要脂肪酸为 C（29%）、Cω9（18%）和异 C（17%）。基因组 DNA 的 G + C 含量为 50.64 mol%。基于 16S rRNA 基因序列的系统发育分析表明，马赛 - P3275 菌株与 科的成员相关（序列相似性在 90.32 - 92.92%之间），并与单特异性属 （模式种 ）形成一个进化枝。基于这些系统发育和表型差异，马赛 - P3275 菌株被归入一个新属 ，即 属，新种。模式菌株为马赛 - P3275（=CSUR P3275 = CECT 9677）。
