Paliard X, Malefijt R W, de Vries J E, Spits H
UNICET, Laboratory for Immunological Research, Dardilly, France.
Nature. 1988 Oct 13;335(6191):642-4. doi: 10.1038/335642a0.
CD4 and CD8 antigens are simultaneously expressed on most of the cortical thymocytes, that weakly express the T-cell antigen receptor(TCR)/CD3 complex. Mature peripheral T cells, however, strongly express the TCR complex and are positive for either CD4 or CD8. Nevertheless, a small percentage of peripheral CD3+ T cells express CD4 and CD8 simultaneously. These mature, double positive cells could be intermediates between CD4+CD8+ thymocytes and mature, single positive T cells, or they may originate from single positive T cells that acquire either CD4 or CD8. Here we report that activation and culturing of cloned CD4+ T cells in interleukin-4 (IL-4), results in the acquisition of CD8 due to its de novo synthesis. The IL-4-induced co-expression of CD8 on CD4+ T cells is reversible, in that CD8 disappeared from double positive T-cell clones isolated in IL-4, when they were cultured in IL-2. CD8 induced by IL-4 can be functional as a monoclonal antibody to CD8 inhibited anti-CD3-mediated cytotoxicity by a double positive T-cell clone.
CD4和CD8抗原在大多数皮质胸腺细胞上同时表达，这些细胞弱表达T细胞抗原受体（TCR）/CD3复合物。然而，成熟的外周T细胞强烈表达TCR复合物，且CD4或CD8呈阳性。尽管如此，一小部分外周CD3 + T细胞同时表达CD4和CD8。这些成熟的双阳性细胞可能是CD4 + CD8 +胸腺细胞与成熟的单阳性T细胞之间的中间体，或者它们可能源自获得CD4或CD8的单阳性T细胞。在此我们报告，在白细胞介素-4（IL-4）中激活并培养克隆的CD4 + T细胞，会由于其从头合成而导致获得CD8。IL-4诱导的CD4 + T细胞上CD8的共表达是可逆的，即当在IL-2中培养时，在IL-4中分离的双阳性T细胞克隆中的CD8消失。IL-4诱导的CD8可发挥功能，因为针对CD8的单克隆抗体可抑制双阳性T细胞克隆的抗CD3介导的细胞毒性。
