Sandhi Ramandeep K, Pothula Ratnasri, Pothula Satyendra K, Adams Byron J, Reddy Gadi V P
Department of Research Centers, Western Triangle Agricultural Research Center, Montana State University , 9546 Old Shelby Rd., P.O. Box 656, Conrad, MT 59425.
Department of Biology, Monte L. Bean Museum, and Evolutionary Ecology Laboratories, Brigham Young University , Provo, UT 84602.
J Nematol. 2020;52:1-11. doi: 10.21307/jofnem-2020-060.
A total of 30 different agricultural fields in the Golden Triangle Region of Montana, USA were surveyed, and 150 soil samples were evaluated for the presence of entomopathogenic nematodes (EPNs). The authors isolated EPNs from 10% of the collected samples. The recovered isolates were identified as and by using morphological and molecular analysis. was found from two fields, Kalispell ( 1) and Choteau ( 2). (1 and 2) differed significantly from each other in terms of morphological characters for infective juveniles (distance from anterior end to excretory pore and nerve ring) and 1st generation males (body length, spicule length, gubernaculum length, oesophagus, tail, and anal body diameter). 2 and were recovered from the same field in Choteau. All these species were recovered from wheat fields with sandy clay loam and loam soils with 3.3 to 3.4% organic matter content and pH 8. A total of 30 different agricultural fields in the Golden Triangle Region of Montana, USA were surveyed, and 150 soil samples were evaluated for the presence of entomopathogenic nematodes (EPNs). The authors isolated EPNs from 10% of the collected samples. The recovered isolates were identified as and by using morphological and molecular analysis. was found from two fields, Kalispell ( 1) and Choteau ( 2). (1 and 2) differed significantly from each other in terms of morphological characters for infective juveniles (distance from anterior end to excretory pore and nerve ring) and 1st generation males (body length, spicule length, gubernaculum length, oesophagus, tail, and anal body diameter). 2 and were recovered from the same field in Choteau. All these species were recovered from wheat fields with sandy clay loam and loam soils with 3.3 to 3.4% organic matter content and pH 8.
对美国蒙大拿州金三角地区的30个不同农田进行了调查，并对150份土壤样本进行了昆虫病原线虫（EPNs）检测。作者从10%的采集样本中分离出了EPNs。通过形态学和分子分析，将回收的分离株鉴定为[具体名称1]和[具体名称2]。在两个田地中发现了[具体名称1]，分别是卡利斯佩尔（1个样本）和乔托（2个样本）。[具体名称1]（1个样本和2个样本）在感染性幼虫的形态特征（前端到排泄孔和神经环的距离）以及第一代雄虫（体长、交合刺长度、引带长度、食管、尾部和肛门体直径）方面彼此有显著差异。[具体名称2]和[另一具体名称]是从乔托的同一个田地中回收的。所有这些物种都是从含有3.3%至3.4%有机质且pH值为8的砂质粘壤土和壤土的麦田中回收的。对美国蒙大拿州金三角地区的30个不同农田进行了调查，并对150份土壤样本进行了昆虫病原线虫（EPNs）检测。作者从10%的采集样本中分离出了EPNs。通过形态学和分子分析，将回收的分离株鉴定为[具体名称1]和[具体名称2]。在两个田地中发现了[具体名称1]，分别是卡利斯佩尔（1个样本）和乔托（2个样本）。[具体名称1]（1个样本和2个样本）在感染性幼虫的形态特征（前端到排泄孔和神经环的距离）以及第一代雄虫（体长、交合刺长度、引带长度、食管、尾部和肛门体直径）方面彼此有显著差异。[具体名称2]和[另一具体名称]是从乔托的同一个田地中回收的。所有这些物种都是从含有3.3%至3.4%有机质且pH值为8的砂质粘壤土和壤土的麦田中回收的。
