Sanchez WinDi, Shapiro David, Williams Geoff, Lawrence Kathy
Department of Entomology and Plant Pathology, Auburn University, Auburn, AL, 36849.
209 USDA-ARS, SEA, SE Fruit and Tree Nut Research Unit, 21 Dunbar Road Byron, GA, 31008.
J Nematol. 2021 Jul 8;53. doi: 10.21307/jofnem-2021-063. eCollection 2021.
The goal was to determine the efficacy of entomopathogenic nematodes (EPNs) on small hive beetle (SHB) in Alabama soils. The objectives were to (i) determine the pupation success of SHB wandering larvae; (ii) determine the efficacy of EPNs on SHB wandering larvae in natural and autoclaved soil; and (iii) determine the efficacy of EPNs on SHB wandering larvae in three Alabama soil types at typical low moisture levels. The Alabama soils were Kalmia loamy sand (KLS), Benndale fine sandy loam (BFSL), and Decatur silt loam (DSL). , , S. , and were tested at population densities of 5, 10, 20, 40, and 80 third-stage infective EPN juveniles (IJ3) per 130 cm soil. Pupation success in SHB population densities of 5, 10, and 20 wandering larvae per Petri dish were similar. Of the six EPN species, achieved the highest efficacy across all EPN population densities in both natural and autoclaved soil. and achieved the next highest efficacies; however, they were significantly less effective than parasitized 87% SHB wandering larvae across all population densities tested. achieved the best efficacy colonizing 94% of the SHB in the KLS soil, 80% in the BFSL soil, and 47% in the DSL soil. In conclusions, is be a promising biological control EPN to implement into a management system on SHB.
目标是确定昆虫病原线虫（EPNs）对阿拉巴马州土壤中小蜂螨（SHB）的防治效果。具体目标如下：（i）确定SHB漫游幼虫的化蛹成功率；（ii）确定EPNs对自然土壤和高压灭菌土壤中SHB漫游幼虫的防治效果；（iii）确定EPNs在典型低湿度水平下对阿拉巴马州三种土壤类型中SHB漫游幼虫的防治效果。阿拉巴马州的土壤分别是卡尔米亚壤质砂土（KLS）、本代尔细砂壤土（BFSL）和迪凯特粉质壤土（DSL）。斯氏线虫、异小杆线虫、小卷蛾斯氏线虫和嗜菌异小杆线虫在每130厘米土壤中5、10、20、40和80条第三阶段感染性EPN幼虫（IJ3）的种群密度下进行了测试。每个培养皿中5、10和20只SHB漫游幼虫的种群密度下化蛹成功率相似。在六种EPN物种中，斯氏线虫在自然土壤和高压灭菌土壤中的所有EPN种群密度下均表现出最高的防治效果。异小杆线虫和小卷蛾斯氏线虫的防治效果次之；然而，它们的效果明显低于斯氏线虫，在所有测试的种群密度下，斯氏线虫能寄生87%的SHB漫游幼虫。嗜菌异小杆线虫在KLS土壤中防治效果最佳，能定殖94%的SHB，在BFSL土壤中为80%，在DSL土壤中为47%。总之，斯氏线虫有望成为一种可应用于SHB管理系统的生物防治EPNs。
